Expression Of EAAT MGluR And TIMP In Brain Tissues Of Rats With Focal Cerebral Is Chemai

Research background: The cerebrovascular disease is the common and frequently- occurring disease which threaten to the human life and health. In recent years, with the increased incidence rate of anemic cerebrovascular disease, it is urgent and more important to study the pathogenesis of cerebrovascular disease and to find the safe and effective substance to control it.Objective: To determine the mRNA expression of EAAT,mGluR and TIMP in brain tissues of rats with focal cerebral ischemia and to study pathogenesis of brain injury induced with focal cerebral ischemia.Methods: model of rat with focal cerebral ischemia was established with the method of middle cerebral artery occlusion, the brain infarct area and water content was measured, and the mRNA expression of EAAT1, 2, 3 and mGluR4, 6 and TIMP1, 2 in brain tissues was also analyzed with RT-PCR method.Results: it was found the obvious pathological alteration in brain tissues of ischemic rats, and the infarct area and water content also increased (p<0.05). The EAAT-1, EAAT-2, EAAT-3 mRNA expression increased significantly in the brain tissure of rat with ischemic injury, and there is no changes of mGluR-4 and mGluR-6 mRNA expression in ischemic cerebral tissues. TIMP1, TIMP2 mRNA expression also increased in ischemic cerebral tissues.Conclusions: The up-regulation of EAAT1, EAAT2 and EAAT3 mRNA is resulted from the compensatory enhanced function of normal nerve cells and prevent it from the excitotoxic injury of glutamate.The increased TIMP1 and TIMP2 mRNA is also belong to the compensatory response, it can inhibit the expression of MMP and prevent brain tissues from the injury by interrupting the interstitial degradation with MMP, it also limits the spread of the inflammatory mediators. It is also suggested that the high expression of TIMP1 and TIMP2 can protect the cells from the injury of inflammation process.