To Investigate The Expression And Significance Of Leptin And Its Receptor(ob-R) In Primary Hepatic Carcinoma(PHC) With Tissue Microarray

OBJECTIVE To detect the expression and significance of leptin and ob-R in PHC, meanwhile to contrast MVD₁(representing CD34) and MVD₂(representing CD105) for investigating the role of leptin and its receptor in angiogenesis in hepatoma;To detect the distinction of expression of ki67 between hepatoma and paracarcinoma tissue and to investigate the role of leptin and its receptor in proliferation in hepatoma.METHOD The expression ofleptin and its receptor,CD34,CD105 andKi67 in 76 cases hepatoma,53 cases paracarcinoma tissue were examined by immunohistochemistry PV9000 and tissue microarray technique,and by SPSS 11.0 statistical analysis package analyze the results.RESULTS 12 strong positive cases(accounting 22.36%),22 moderate cases (accounting 28.95%),27 weakly cases(accounting 35.53%) of leptin in 76 cases of heptoma;46 strong and moderate cases(accounting 86.79%),7 weakly cases in 53 cases paracarcinoma tissue.Meanwhile the expression of leptin represented predominant discrepancy between cancer and paracarcinoma tissue(x²=44.58,p＜0.05).The expression of ob-R was 17 strong positive cases(accounting 22.36%),22 moderate cases(accounting 28.95%) and 27 weakly cases(accounting 35.53%) in hepatoma.Meanwhile the expression of ob-R represented predominant discrepancy between cancer and paracarcinoma tissue(x²= 18.55,p＜0.05).The expression of leptin in hepatoma had no statistical significance with acromegalic ages,tumor's diameter,clinic accompaniment diseases,differentiated degree and the discrepancy represented no significant(P＞0.05);But it represented statistical significance with patient's gender(x² = 119.92 p＜0.05).The expression of ob-R in hepatoma had no statistical significance with patient's ages,tumor's diameter,clinic accompaniment diseases,defficientiated degree and the discrepancy represented no significant(P＞0.05);But it represented statistical significance with patient's gender(x²= 144,P＜0.05).The express intension of between leptin and MVD₁ represented negatively correlation(r=-0.323,p＜0.05),so as MVD₂(r=-0.309,p＜0.05).The expression of MVD₂ was weaker than the expression of MVD₁,at the same time they represented significant difference between them(t=3.746,p＜0.05).The express intension of between leptin and ki67 represented negatively correlation(r=-0.282,p＜0.05).CONCLUSION The expressions of leptin and ob-R in HCC were all weaker than that in paracarcinoma tissues,it indicated that leptin and ob-R may have determinated inhibitory action in carcinogenesis and development of HCC.The expression of leptin and ob-R in HCC had no obviously relationship with patient's ages,tumor's diameter,clinic accompaniment diseases,differentiated degree,but had pridominent statistical significance with acromegalic gender.,it indicated that gender may affect the express level of leptin and ob-R in hepatoma.The expression of MVD₁ and MVD₂ represented negatively correlation with the express intension of leptin and the expression of MVD₂ was weaker than the expression of MVD₁,these indicated leptin may inhibit the angiogenesis of hepatomilia cell.The expression of ki67 represented negatively correlation with increasing for the express intension of leptin between HCC and paracarcinoma tissue,these indicated leptin may inhibit the proliferation ofhepatomilia cell by some kind of pathway.