The Effect Of Using Omega-3 PUFA At Tumor Rats

Background:malignant tumor is an obstinate illness of modern medicine,and the body consumption caused by cancer is one of its pathognomonic features,and also is the main reason which leads patients' death.Protein loss is most important in cancer induced body consumption,because proteins are not only nutritive material,but also play an important role in many vital movements.Recent years,many researches show thatω-3 PUFA could inhibit tumor growth and improve cancer induced body consumption.There is few internal researches aboutω-3 PUFA therapy tumor,especially by the per-vein way.Objective:This research administer tumor rats with omega-3 PUFA or omega-6 PUFA emulsion per-jugular vein to identify the effect of using omega-3 PUFA in oncotherapy.Methods:We randomly divide 40 SPF SD Rats into 4 groups at 10 of each-group,named normal group,tumor group,ω-3 PUFA group andω-6 PUFA group.Inoculate the last three groups with walker 256 sarcoma cell to set an animal model.After 4 days we administerω-3 PUFA group andω-6 PUFA group with corresponding fat emulsion,keep on 10 days. At the end of the 14 day experiment stage,we kill all 40 rats,gather blood and muscle.We determine the concentration of IL-6,TNF-αand albumin in serum.And determine the Atrogin-1 gene protein expression by Western Blot.During the 14 day experiment stage we weight these rats on alternate day.Than compare the different effect of this tow fat emulsion.Results:the weight without ascitic fluid ofω-3 PUFA group is heavier than tumor group (186.10±3:8.43g vs 174.80±8.61g;P=0.022),and is no statistical difference withω-6 PUFA group(186.10±8.43g vs 178.00±9.92g;P=0.094).There is no statistical difference between tumor group andω-6 PUFA group's weight without ascitic fluid(P=0.501).The weight loss ofω-3 PUFA group is less than tumor group(-21.30±2.95g vs -26.80±1.03g;P=0.009), and is no statistical difference withω-6 PUFA group(-21.30±2.95g vs -24.7±3.97g; P=0.094).There is no statistical difference between tumor group andω-6 PUFA group's weight loss(P=0.295).The ascitic fluid ofω-3 PUFA group and tumor group is no statistical difference(43.40±2.76g vs 44.10±2.18g;P=0.470),and it is same toω-6 PUFA group and tumor group's ascitic fluid(45.70±2.45g vs 44.10±2.18g;P=0.104).But the ascitic fluid ofω-3 PUFA group is less thanω-6 PUFA group(P= 0.022).The albumin ofω-3 PUFA group is higher thanω-6 PUFA group,but it's not statically significant (27.07±1.40 g/L vs 26.17±3.44 g/L;P= 0.453).The serum IL-6 concentration ofω-3 PUFA group is lower thanω-6 PUFA group(56.70±41.79PG/ml vs 141.72±98.32PG/ml; P=0.002),and is no statistical difference with tumor group(56.70±41.79PG/ml vs 71.63±39.50PG/ml;P=0.501).Butω-6 PUFA group's serum IL-6 concentration is higher than tumor group(P=0.010).The serum TNF-αconcentration ofω-3 PUFA group is lower thanω-6 PUFA group(131.67±22.10PG/ml vs 167.26±37.98PG/ml;P=0.007),and no statistical difference with tumor group(131.67±22.10PG/ml vs 141.27±19.37PG/ml; P=0.441).Butω-6 PUFA group's serum TNF-αconcentration is higher than tumor group (P=0.042).The Atrogin-1 gene protein expression ofω-3 PUFA group is lower thanω-6 PUFA group.Conclusions:1.comparing toω-6 PUFA,ω-3 PUFA can reduce the serum concentration of IL-6 and TNF-αin tumor rats by inhibit the NF-κB active-pathway.2.comparing toω-6 PUFA,ω-3 PUFA can increase the des- ascitic-fluid-weight and improve the weight loss of tumor rats.3.ω-3 PUFA can down regulate the protein expression of Atrogin-1 gene in tumor rats' muscle,to hint there may be existing some possible mechanism influence the ubiquitinproteasome system,thus improve the body protein wasting.