| Background and Aims 2-Octyl Cyanoacrylate is a kind of chemical tissue adhesive, it have many functions in adhesive capacity, mechanics and biology. When exposed to water or blood, it can have powerful adhesion instantly. With the technical advancement of tissue adhesive, the adhesive used by 2-Octyl Cyanoacrylate have been devlopped quickly.Because its nice biocompatibility, it hve been many successful clinical applications in forensic sursery, brain sursery, aural sursery, gynaecology and obstetrics, facial organs, plastic surgery and etc.Because the tissue adhesive is convenient, quick, safe and inexpensive than others, it has been used in helcoma, strabotomy, anophthalmic cavity reconstruction, and so on. However, no article is showed that the tissue adhesive has been used in corneal and sclerotic chapped pentrating wounds.The experimental study investigated the feasibility of agglutinating corneal and sclerotic chapped pentrating wound with the tisuue adhesive, and evaluated the biocompatibility to ocular surface. Based on these facts, we study the effect of agglutinating corneal and sclerotic chapped pentrating wound. Our study provided experiment foundation for tissue adhesive to apply in ophthalmology, and a new idea of progress in ophthalmology operation and new surgery carried out. The tissue adhesive will have wide prospect in ophthalmology in the future.Part 1 the experimental study on the histocompatibility of tissue adhesive and application for corneal chapped petrating woundObjective: To investigate the biocompatibility to ocular surface with CA and the application for corneal chapped petrating wound.Methods:1. 45 Japanese white rabbits are random divided 5 groups. Each groups had 9 rabbits. 2. all the rabbits's left eyes are sutural group, Right eyes are CA group. On days 3, 7, 14, 30, 90 after surgery operation, rabbits are executed by mainline, then take their eyeballs and corneas to observe the experimental targets.3. on the time after operation, we observe the two groups of corneal wound, nubecula, new blood vessels of cornea, anterior chamber, degradation of CA, protein content of aqueous humor, corneal endothelial cell by Microscope, tension of eyeball, light microscope, transmission electron microscope.Results:1. Surgury time of CA group less than sutural group.2. On day 3 after operation, two groups have part of corneal turbidity.on day 7, new blood vessels of CA group more than sutural group. On day 30, the vessels of sutural group are disappeared, CA group have 2-3 blood vessels. On day 90, all of blood vessels have been absorbed, CA have fall out, sutural group have been seen white sutural trail.3. After operation, no difference has been observed with two groups by protein content of aqueous humor and corneal endothelial cell by Microscope. On each observing point, tension of two groups'eyeball more than the normal, and the tension increase gradually with increasing time after surgery.4. Forepart after surgery operation, by light microscope, corneal wound of sutural group have inflammatory cell infiltration and corneal epithelium implantation; corneal wound of CA group have infalmmatory cell infiltration a little, and no corneal epithelium implantation. on day 30, fibroblasts and inflammatory cells of two groups decrease distinctly, and fiber cells increase. On day 90, the wound of two groups cicatrize fully. By transmission electron microscope, the ultrastructures of corneal endothelial cells have no difference between the two groups.Conclusion:1. CA has better biocompatibility to ocular surface, and don't bring toxicity to eyes and no pathology change.2. CA can quickly close corneal chapped petrating wound, and bring enough tensile force that sustain the wound to close.3. When corneal wound closing with CA, cornea can make some new blood vessels. But with the wound closed, the vessels disappeared completely. 4. CA can agglutinate the corneal wound conveniently, quickly, economically, and avoid the hypo-damnify because of sutural operation.Part 2 the experimental study on the application for corneal chapped petrating woundObjective: To compare that CA treat the sclerotic chapped pentrating wound with sutural operation, investigate the feasibility of application with CA.Methods:1. 45 Japanese white rabbits are random divided 5 groups. Each groups had 9 rabbits.2. All the rabbits's left eyes are sutural group, Right eyes are CA group. On days 3, 7, 14, 30, 90 after surgery operation, rabbits are executed by mainline, then take their eyeballs to observe the experimental targets.3. On the time after operation, we observe the two groups of sclerotic wound, conjunctiva injection, ocular protomerite and deutomerite, operation time, tension of eyeball, light microscope, transmission electron microscope.Results:1. Surgury time of CA group is less than sutural group.2. On each time after operation, two groups have no inflammation in ocular protomerite and deutomerite, and no difference on the conjunctiva injection.3. Each observing point after operation, tension of two groups'eyeball more than the normal, and the tension increase gradually with increasing time after surgery.4. Forepart after surgery operation, by light microscope, CA group mechanocyte of sclerotic wound close from inside to outside. Sutural group is contrary. On day 30, fibroblasts and inflammatory cells of two groups decrease distinctly, and fiber cells increase. On day 90, the wound of two groups cicatrize fully. By transmission electron microscope, sclerotic endothelial cells of two groups have no difference.Conclusion:1. CA can quickly close sclerotic chapped petrating wound, and bring enough tensile force that sustain the wound to close.2. CA has better histocompatibility with sclerotic wound, and don't bring toxicity to sclera andretina. CA doesn't bring pathology change. 3. On forepart the phenomenon that CA treat sclerotic wound is different with suture treating, but these don't affect the rate of wound close.4. CA can agglutinate the sclerotic wound conveniently, quickly, economically, and avoid the hypo-damnify because of sutural operation.
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