Corretation Study On Endophyte And Quality Of Rhizoma Chuanxiong

Rhizoma Chuanxiong is dry rhizomes of Umbelliferae plant Ligusticum chuanxiong Hort..It is a famous chinese traditional medicinal crop and its main producing area is Sichuan.It can promote Qi and blood to circulate,dispel wind and restrain pain.Research results in near years indicated that the volatile oil was the main active component,which had strong physiological activity.There were some differences at chemical components and content between different habitations and varieties of Ligusticum chuanxiong Hort.The formation of famous-region of Ligusticum chuanxiong attribute to the specific soil and climate in ecological environment.It is hard to explain quality difference in different micro-environment using method of macroscopical ecology.Therefor,we study the corretation between micro-environment and quality.Firstly,87 strains endophytic fungi were isolated from the rhizomes of Ligusticum chuanxiong Hort.and they were morphologically identified belonging to 17 genera,4 families,3 orders and 1 classe.Moniliales,which includes 94.3%of the whole strains,is believed the dominant order.While Fusarium,Aspergillus, Cephalosporium,Ovulariopsis are believed the dominant genera,which may include 30.0%,19.5%,14.9%,14.9%of the whole strains.Results firstly revealed the differences of number,distribution,and composition from vary endophytic fungi of Ligusticum chuanxiong Hort..At first time,the diversity of endophytic fungi in the main areas is better than others;.endophytic fungi from different areas have their special population,for esample,the endophytic fungi of Shiyan is stable.After DNA was extracted directly from Radix Ligusticum chuanxiong,sepefic primers were used to amplify the object gene 18S rDNA,then the object gene was partially restricted.The ARDRA figure showed that difference exist in different samples,which suggested kinship and genetic diversity of entophytic fungi. Entophytic fungi can only get 1 or 2 bands by DGGE,which maybe comfirm DGGE is not good at test product over 500bp.Secondly,We used DGGE to study endogenetic bacteria.DGGE showed 38 bands with 16S rDNA product separated.The different samples have different type and quantity bands.There are common and specific bands,which illuminats that different habitations and varieties possess similar and specific microorganism population.Thirdly,48 chemical components were isolated and identified from the volatile components.Chemical components and content of 6 samples were different and Ligustilide is the main component.PAC indicated that the 5 principal components contained 21 chemical composition,and the accumulated dedication rate is up to 100%,which includes all volatile components in principle.Cluster analysis showed that the volatile components is corretate with habitations.Meanwhile,the oil of Ligusticum chuanxiong in different habitats contain different fatty acids range from 13.69%to 38.38%.Finally,we did correlation analysis among endogenetic fungi,endogenetic bacteria and volatile components in oil of Ligusticum chuanxiong,by SPSS.And results concluded that the existence of Ovulariopsis had positive correlation with content of Ligustilide,in contrast the existence of Penicillium and Wardonmyces had negative correlation with content of Ligustilide.Datas from endogenetic fungi and chuanxiong oil of Ligusticum chuanxiong in Aoping and Shiyang from 2006 to 2007 indicated the existence of Ovulariopsis could promote production of Ligustilide.There was no correlation between endogenetic bacteria and volatile components,but its population constitution had correlation with fatty acids,which suggested endogenetic bacteria had relationship with synthesis of plant primary metabolite.Our research can give some references from a new degree measure to explain the formation of famous-region of Ligusticum chuanxiong.