Study On Relationship Between Cystic Fibrosis Trans-membrane Conductance Regulator And Pathogenesis Of Ovarian Hyperstimulation Syndrome

Objective: To explore the relationship between cystic fibrosis transmembrane conduc- tance regulator (CFTR) and the pathogenesis of ovarian hyperstimulation syndrome (OHSS).Methods: Immature female Wistar rats aged 22 days were divided into two groups: the OHSS group (16 rats) was given 10 IU PMSG for 4 consecutive days and given 30 IU hCG on 26-day-old to produce ovarian hyperstimulated manifestations; the conventional stimulation group (16 rats) was given 10 IU PMSG on 24-day-old and given 10 IU hCG on 26-day-old. On 28-day-old, rats were injected Methylene blue dye solution via the tail vein to evaluate the capillary permeability in the peritoneal cavity and ovary; blood samples were collected from the inferior caval vein to evaluate serum E2 level; the expression of CFTR protein in rat ovaries was examined by Immunohistochemistry and Westem-blotting respectively.Results: The peritoneal Methylene blue concentration and ovarian Methylene blue content were significantly higher in the OHSS group; ovarian wet weights, serum E2 level and ovarian CFTR protein expression were also markedly increased in the OHSS group than the conventional stimulation group(P <0.05).Conclusion: Estradiol-induced abnormally high CFTR expression probably result in formation of ovarian cysts and electrolyte disturbances during ovarian hyperstimulation syndrome.