Coaction Of CFTR And AQP1in Permeability Of Body Fluid In Ovarian Hyperstimulation Syndrome

Part Ⅰ Coaction of CFTR and AQP1increases permeability of peritoneal epithelial cells on estrogen-induced ovarian hyperstimulation syndromeObjective:The present study was designed to investigate the coactions of cystic fibrosis transmembrane conductance regulator (CFTR) and aquaporin1(AQP1) on permeability of peritoneal epithelial cells on estrogen-induced ovarian hyperstimulation syndrome.Materials and methods:Ovarian hyperstimulation syndrome (OHSS) was induced by injection of human menopausal gonadotropin (HMG) plus human chorionic gonadotropin (hCG). Using western blot and short-circuit current (Isc) techniques, we investigate the potential coactions of analysis in CFTR and AQP1on the hyperpermeability of body cavity peritoneal epithelial cells in the pathogenesis of OHSS.Results:HMG plus hCG induced OHSS in rats and up-regulation expression of CFTR and AQP1in peritoneal epithelial cells. Using Isc techniques, we investigate the potential coactions of analysis in CFTR and AQP1on the hyperpermeability of body cavity peritoneal epithelial cells in the pathogenesis of OHSS. The rats develop OHSS symptoms, with up-regulated both CFTR and AQP1expression and enhanced CFTR channel activity in peritoneal epithelial cells, can also be mimicked by administration of estrogen, alone in ovariectomized rats.Conclusion:This study confirms the coactions of CFTR and AQP1play a critical role in the development and progression of increased peritoneal permeability in severe OHSS. These findings may provide grounds for ameliorating assisted reproduction treatment strategy to reduce the risk of OHSS and improve in vitro fertilization (IVF) outcome. Part Ⅱ The treatment of ovarian hyperstimulation syndrome based on CFTR and AQPlfrom animal studyObjective:The present study was designed to investigate the treatment of ovarian hyperstimulation syndrome (OHSS) based on cystic fibrosis transmembrane conductance regulator (CFTR) and aquaporin1(AQP1) from animal experiment.Materials and methods:Ovarian hyperstimulation syndrome (OHSS) was induced by injection of human menopausal gonadotropin (HMG) plus human chorionic gonadotropin (hCG). Using western blot and short-circuit current (Isc) techniques, we investigate the effect of treatment based on CFTR and AQP1on the hyperpermeability of body cavity peritoneal epithelial cells in the pathogenesis of OHSS. Injection of progesterone suppresses CFTR activity, OHSS symptoms as well as CFTR and AQP1expression. Besides, the Isc results showed that AQP1inhibitor, HgCl2, can suppress CFTR channel activity. Therefore, antisera against CFTR or AQP1intraperitoneal injection to OHSS animals result in alleviation of the symptom.Results:HMG plus hCG induced OHSS in rats. Administration of progesterone suppresses CFTR activity, OHSS symptoms as well as CFTR and AQP1expression. Besides, AQP1inhibitor, HgCl2, can suppress CFTR channel activity. Therefore, antisera against CFTR or AQP1to OHSS animals result in alleviation of the symptom.Conclusion:This study confirms the treatment based on CFTR and AQP1have a obvious effect on the development and progression of increased body cavity peritoneal epithelial permeability in severe OHSS. These results from animals may suggest measures for ameliorating assisted reproduction treatment strategy to reduce the risk of OHSS and improve in vitro fertilization (IVF) outcome.