The Expanding Process And Quality Research Of Radix Polygoni Multiflori

Radix Polygoni Multiflori is the dried root of the polygonaceae plants Polygonμm Multiflorμm Thunb.,Radix Polygoni Multiflori used as traditional chinese medicine in the past dynasties.There are some differences in efficacy between raw herbs and its processed products.The raw herbs have the effects to relax the bowelss and relief the ulcer,but with toxicity.After processing,the chemical constituents of the products cause the changes of its pharmacological action,decreasing the purging activity,enhancing the invigoration,nourishing liver and kidney,supplementing essence and blood,blackening beard and hair. There are a lot of methods of the traditional processing of Radix Polygoni Multiflori,but all of them have some disadvantages,such as:wasting time and energy,complex operation,low mechanization degree,quality depending on to experience and being unsuitable continuous production.According to food extrusion principle,Radix Polygoni Multiflori has been extruding processed,and its quality before and after processing has also been researched in this paper,so as to study the influence of the expanded process on the main effective components of Radix Polygoni Multiflori.In the experiment,we use expanded machine for the expanded process,and give Radix Polygoni Multiflori and expanded Radix Polygoni Multiflori a conventional identification,including morphological identification,microscopic identification, TLCidentification and so on,determining the expansion rate of Radix Polygoni Multiflori by a colorimetric method,determining the content of polysaccharide, free and combined anthraquinone in both Radix Polygoni Multiflori and expanded Radix Polygoni Multiflori by colorimetric method,determining the content of the free and combined emodin and physcion,the stilbene glucoside in both uberfleeceflowers.The results are as follows:1.Microscopic,TLCidentification:After expanded,the organizational structure of Radix Polygoni Multiflori changed,and starch grain gelatinization,reduced; most cluster crystal destructed;catheter broken;cork cell broken;solid structure got soft;the nμmber of TLC spot and Rf value are same between beforeand after expansion.2.The detection of the extrusion rate:by the means of pushing the volμme,take both Radix Polygoni Multiflori and expanded Radix Polygoni Multiflori of the same weight,then put them in the beaker full of radish zi separately,the volμme they pushed is the volμme of the radish zi,so that we could get the extrusion rate, the result showed that the extrusion rate is 8.61.3.Content determination:We detect the content of polysaccharide of both Radix Polygoni Multiflori and expanded Radix Polygoni Multiflori by phenol-sulfuric acid colorimetric method,and give the extraction condition an optimization in heating temperature,heating time,ice bath time.Finally we determined the conditions that determined solution was put into boiling water bath for 30 mintunes,and then taken out,put into ice bath for 20 mintunes,the corresponding phenol-acetate magnesiμm as blank,determining the absorbance at 487.00nm.The result showed that the content of polysaccharide of expanded Radix Polygoni Multiflori increased.4.The content of the free and combined anthraquinone in both Radix Polygoni Multiflori and expanded Radix Polygoni Multiflori had been detected by acetate magnesiμm-methanol colorimetric method,magnesiμm-methanol as blank, determining the absorbance at 510nm.The result showed that the content of the free and combined anthraquinone both decreased,especially the latter.5.The content of the free and combined emodin and physcion in both Radix Polygoni Multiflori and expanded Radix Polygoni Multiflori had been detected by HPLC with methanol-0.1%phosphoric acid(V= 85:15 ) elution,flow rate was 1.0ml/min,the detection wavelength was 254 nm.The result showed that the content of the free and combined emodin and physcion all decreased after expanded.6.The content of the stilbene glucoside in both Radix Polygoni Multiflori and expanded Radix Polygoni Multiflori had been detected by HPLC with acetonitrile-water(V = 25:75 ) elution,flow rate was 0.6ml/min,the detection wavelength was 320 nm.The result showed that the content of the stilbene glucoside slightly decreased after expanded.