Analysis Of Diabetic Characteristic Metabolites And Hypoglycemic Drug Toxicity Using NMR-based Metabonomics

With the improvement of people's standard of living and lifestyle changes, the incidence of diabetes showed increasing year by year, the trend has been more and more people's attention. However, because diabetes is a metabolic disorder as a whole which involved multiple factors, the regulation mechanism is very complicated. Current research methods neglected dynamic metabolic changes of organ in the overall, and can not provide the overall cell function information. Therefore, it is necessary to find a new way to get biomedical information of diabetes in organisms as a whole, metabonomic approach which can get overall information from systematic perspective provides a good technology platform for the study.As a non-invasive and efficient means of detection, NMR (nuclear magnetic resonance) technology is commonly used metabonomic methods. The NMR-based metabonomic approach has been widely used in biofluids research. The aim of this paper is to find characteristic metabolites of diabetes, analyse the toxicity of vanadium compounds by NMR-based metabonomic approach, and explore the possibility of methods used in diabetes research. The main contents of this study are as follows:First, by NMR-based metabonomic approach, obtaining NMR spectrum of blood, urine from animal model and human; finding characteristic metabolites of diabetes with pattern recognition analysis, and building experimental research methods of NMR-based metabonomics .Second, on the basis of inspecting hypoglycemic effect of vanadium compounds , analyzing toxicity of VOSO₄ by NMR-based metabonomic approach, understanding toxic effects of vanadium compounds, and deepening the understanding of vanadium compounds.Third, with substantive literature, making a brief overview of NMR-based metabonomic approach in diabetic research and providing prospects to follow-up study. In short, NMR-based metabonomics approach can be used for the distinction between diabetes and normal control, and drug toxicity analysis, it is an effective way to find characteristic biomarker and will have a broad prospects of development in the diagnosis, pathological research of diabetes in the future.