| The rupture of Aneurysm is the most common reason for subarachnoid hemorrhage(SAH), the average incidence of subarachnoid hemorrhage is 5-20 per 100000 annually. The principle of treatment for aneurysm is to completely exclude it from the blood circulation. And completely occludee the aneurysm. The most of aneurysms can be treated using endovascular treatment method, which use the intervention method to treat cerebral aneurysm. Recently, with the development of material science,molecular biology and Tissue Engineering, the treatment of aneurysm through multi-discipline gradually show important value and bright future.This experiment used the FB and alginate as the basic materials to design tissue engineering embolic material for the treatment of intracranial aneurysm. In this research, fibroblasts and Na-alginate hydro-gels are chosen as seeding cells and three-dimensional scaffold respectively. Alginate afforded three-dimensional culture environment for FB. Gradually, fibrous connective tissue substitute degradated alginate hydro-gel, which can occlude the aneurysm for ever. The effect of embolization was determined by histological examination.we explore alginate as a scaffold for cell growth through biological evaluation in vitro. The purpose of this research is to provide a live,non-immune-derived tissue-engineered liquid emblic material for the treatment of cerebral aneurysm.1 the research on the alginate emblazing cerebral aneurysmobjective: explore the feasibility of alginate as embolic material for the treatment of intracranial aneurysm. Methods: The compression tests were conducted with a materialscharacterization Instron machine (INSTRON 5869). The histocompatibility was observed by injecting the alginate into rabbit neck subcutaneous; the better injected method of alginate is determined by aneurysm emblolization experiment in vitro. There were two methods for the injection of alginate, side by side microcatheter and concentric two microcatheter.; the effects of controllability and embolization for the alginate were evaluated by aneurysm embolization experiment in vitro. The aneurysm modle was established using the rabbit carotid artery. Alginate were injected into aneurysm cavity through concentric two microcatheeter made by trocar in experimental group eitht New Zealand white rabbits. The animals were killed in 2 week, 4 week, 2 month, and 4 month, cutting carotid artery and its branches for HE staining to observe the effects of emblization and tissue compatibility of the alginate. The control group were simply ligated the right side of carotid arter distal and killed in 2 week and 4 week to observe the change of vessel cavity by HE staining. The results were analysized by SPSS15.0. results: the compression of alginate gel was stonger than 13kpa, and the volume of alginate gel increased with the concentration fo alginate; there were no significant inflammation reaction in vivo observation; the alginate can be smoothly injected into aneurysm model through the microcatheter; the filling rate of side by side is 71.3±4.9% (minimum 66.0% fill rate, maximum filling rate of 92.5%), and concentric double microcatheter is 86.0±4.2% (minimum 81.1% fill rate, maximum filling rate of 92.5%), the difference was significant (p <0.05). there were no alginate adhere to cather after embolization.alginate successfully occluded the rabbit aneurysm model and no obvious histopathological reaction; only one case in four moths show recanalizaion and the others were completely occluded. Conclusion: the alginate is non adherent liquid embolic material which have good controllability and tissue compatibility and non-toxic material, and could be used for the treatment of intracranial aneurysms.2 the study on the three-dimensional culture of fibroblast cells in alginate gelobjective: explore the growth state of fibroblast cells on alginate gel. The purpose of this research is to provide a live embolic material by tissue engineering technology for the treatment of intracranial aneurysm. Methods: rat skin fibroblast cells were isolated and cultured in vitro. The third generation of cells were chosed as seed cells. 2 volume 4.0% sodium alginate, 1 volume 6mol / L CaCl2 and 1 volume 4×106/ml fibroblasts suspension were mixed, so that final concentration of sodium alginate was 2.0%, CaCl2 was 0.5mol / l, and fibroblasts was 1×106 / m, and were placed in 5% CO2, 37℃saturated humidity incubator for culture. the fibroblast growth state was observed through the inverted microscope, HE staining and scanning electron microscope. Results: just inoculated fibroblasts were round under the inverted microscope, and adhesive to alginate scaffolds. then Fibroblasts began to extend and form many dendrites, like a star, with adhesion to alginate gel more closely. Over time, cells become to be long shuttle type. Occlusion: the fibrolast cells were successfully separated, cultured and amplified from rat skin. the third generation of cell have good shape stability and strong growth; fibroblast cells have good condition in alginate gel, and closely integrated with the alginate gel which is a ideal scaffold material for the culture of fibroblasts .
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