Feasible Study Of Chitosan/gelatin Polymer Film Modified T Tube

Bile duct disease is a very common and frequently occurring disease in general surgery department. It is also a kind of difficult surgery to approach. It has been shown that common bile duct stones have a higher incidence rate in China, accounting for 5%～29%of patients with gallstones, with an average of 18%. Laparoscopic bile duct exploration and stone removal surgery is now considered the best treatment of gallbladder stones and secondary common bile duct lithiasis. The advantage of this surgery is small surgical trauma and minimal abdominal disturbance, however, this also leads to late formation of sinus and extended indwelling of T-tube, which is also the only disadvantage of Laparoscopic Common Bile Duct Exploration (LCBDE) and Bile Duct Stone Extraction.The purpose of this study is to explore whether this technology can be improved through modified T-tube to promote early and strong formation of sinus.[Object] To evaluate the possibility of using modified T-Tube in the sinus formation after LCBDE.[Method]1. Preparation of Chitosan/gelatin polymer film: a certain percentage of chitosan and gelatin mixture was prepared by freeze-drying method.2. Characterization of the film: including evaluation of the membrane thickness, flexibility, HE stained section, microscopic property, in vitro degradation rate and swelling test.3. Toxicity property of the film.4. Rat experiments: rats were implanted with Chitosan/gelatin polymer film. Two weeks later, experimental group and control group were compared by survival rate, sinus pressure measurement and histopathological section.[Results]1. Chitosan /gelatin polymer film is a semi-transparent yellowish Texture flexible membrane with a certain degree of anti-rally.2. Under the microscope, it has a mesh membrane structure, with pore sizes about 80-120μm and thickness around 0.26±0.08mm by micrometer.3. Swelling of the polymer film is between 513-726% in 24 hours 4. Degradation of the polymer film in 15 days is 73.2% and it will take about 25 days for complete degradation, which fits the requirements for body retention of T-tube.5. Toxicology results are within the line of requirements.6. T-tube implantation experiments: Rats were in good condition after implant surgery. In the experimental group and control group, no rats were found dead after 15 days when removing the T-tube. There were no adverse reactions in the both groups.After T-tube was removed, no significant eosinophils and other allergic cells were found in histopathological sections of the surrounding tissues. However comparing with the control group, there was significantly increased fibrous tissue hyperplasia in the modified T-tube to form a complete encapsulation wall. Sinus pressure water test confirmed that, in experimental group, there was early sinus and parcels formation.[Conclusion]1. Chitosan and Gelatin is a biodegradable natural polymer with good biocompatibility.2. It is safe and reliable to use Chitosan/gelatin polymer membrane in vivo.3. Chitosan /gelatin polymer film modified T-tube satisfies the general requirements in the body4. It is a clear advantage that chitosan/gelatin polymer film modified T-tube makes sinus formation5. It still needs further research and development before using Chitosan - gelatin polymer film modified T-tube in the human body.