Extraction, Purification And Application Of 6-Gingerol

6-gingerol is the pungency component in ginger,which has such biological activity as oxidation resistance,mutagenesis resistance,anti-inflammation, anti-rheumatism,anti-kinetosis,antitumor and getting out of the cold.It could be used to produce effective remedy and health food for cerebrovascular disease,theumatic arthritis,gastric ulcer and cancer terminal vomiting.For based on these needs,the 6-gingerol was studied on the extraction,purification and application,and the following result was got:â‘ The fresh ginger juice was adsorbed by marcroporous resin column and polyamide column,then eluted by different concentrations of alcohol.The eluent which contained 6-gingerol was collected and concentrated.The concentrated eluent was extracted by organic solvent.Above 90%purity 6-gingerol was obtained after volatilization.â‘¡Three kinds of organic solvents including ether,n-hexane and chloroform were used to extract it for several times,and 6-gingerol extracted with different organic solvents and different times were determined by HPLC.The optimum extraction processing of 6-gingerol was extracting with 20%ether for two times.â‘¢Through GC-MS,there were 58 kinds flavor material in ginger and 24 kinds in liquid waste from gingerol production.There are same flavor material in two samples by comparative Analysis,but also some flavor material was existed in liquid waste from gingerol production not in ginger.Such as,3,7-Dimethyloct-6-enylacetate, Germacrene,γ-Curcumene,Trans-γ-Bisabolene,Hexadecanocic acid,Nerolidol, Dodecanoic acid,β-Bisabolene,Germacrene,Margaric acid,Ethyl margarate, Dilinoleic acid.This study has provided basis for the effective utilization of flavor in the alcohol liquid waste.â‘£Molecular Template(6-gingerol),function monomer,crosslinking agent, initiatorand solvent were mixed in the reactor.The Molecularly Imprinted Polymer with molecular template 6-gingerol was obtained.After grinded and sieved,the homogeneous particle was obtained.Then molecular template 6-gingerol was eluted by 95%ethanol,and Molecularly Imprinted Polymer which purified 6-gingerol was got.It had so many advantages as strongly selective,large adsorption,well renewable, simple equipment,less investment and low production cost.It was simple,economic, efficient and speedy method to purify 6-gingerol.⑤The content of ginger,alcohol concentration,sugar-acid ratio and glycerine content were designed with the L₉(3⁴) orthogonal experiments.The content of 6-gingerol was the most important factor on the flavor of the ginger wine.While the content of 6-gingerol was 93±2μg/mL,the flavor of the ginger liquor was most tasteful.