Effect Of Triamcinolone Acetonide On The Expression Of Vascular Endothelial Growth Factor In HRPE Cells Induced By Hyoxic Injuries

Objective The aim of this study was to investigate the effects of Triamcinolone acetonide (TA) on the expression of vascular endothelial growth factor (VEGF) in hypoxic injury human retinal pigment epithelial (RPE) cells.Methods Human retinal pigment epithelial cells were cultured to establish the model of hypoxic injury hRPE induced by cobalt chloride.The cultured hRPE cells were divided into 3 groups, including control group, simple injury group, treatment group which was treated with TA and was divided into 4 sub-groups according to the concentration of TA(â‘ 0.05mg/ml,â‘¡0.1mg/ml,â‘¢0.2mg/ml). The releasing rate of LDH in the cultured medium were detected by chromatometry after injury, the expression of VEGF protein was assessed by immunocytochemistry assay, and the VEGF mRNA was studied by reverse transcriptase PCR.Results 1.The human retinal pigment epithelium cells (hRPE-D407 cell line) presented long fusiform or polygon-based shape, monolayer adherence growing. The nucleus was lucency, large and round.The endochylema was abundant.,and the cell boundary was clear. 2.VEGF was detected in normal cultured RPE cells, and its expression increased after hypoxic injuries (P<0.05), the changes of VEGFmRNA paralleled to the changes of its protein.3.The releasing rate of LDH and alterative trend of VEGF in TA treatment groups were decreased compared with hypoxic injury groups (P<0.05).Conclusion The expression of VEGF was increased in hypoxic injury hRPE cells, Triamcinolone acetonide could down-regulate the releasing rate of LDH and the expression of VEGF.