Studies Of Quality Evaluation Of Cirtrus Sp.

For the purpose to study the quality evalution of 10 kinds of crude drugs of Cirtrus sp. , Fructus Aurantii Immaturus,Fructus Auranti,Pericarpium Citri Reticulatae,Pericarpium Citri Reticulatae Viride,Semen Citri Reticulatae),Citrus tangerina Hort.et Tanaka C.erythrosa Tanaka,Folium Citri Reticulatae,Exocarpium Citri Rubrum,Fructus Citri Sarcodactylis,Fructus Citri.To eastablish asystematical quality evalution method of 18 crude drugs of Citrus species including Fructus Aurantii Immaturus,Fructus Auranti,Pericarpium Citri Reticulatae,Pericarpium Citri Reticulatae Viride,Semen Citri Reticulatae),Citrus tangerina Hort.et Tanaka C.erythrosa Tanaka,Folium Citri Reticulatae,Exocarpium Citri Rubrum,Fructus Citri Sarcodactylis,Fructus Citri viride from different areas in China,a HPLC method was set up to determine the contents of synephrine as well as Synenhrine, Hesneridin and Naringin in crude drugs. The chromatographic conditions included an phenomsil c18 BDS(250×4.6mm , 5μ) column ,the mobile phase consisting of synephrine was methanole-water(composed of 0.02% phosphoric acid and 01% sodium dodecyl sulfate)( 7:4),and the mobile phase of flavonone glycosides was methanol-water(composed of 0.02% phosphoric acid and 0.02% triethylamine).The liner ranges of synephrine, hesperidin and naringin were in 1.1320～14.152μg·μL-1,0.2170～2.1680μg·μL-1 and 0.2152～2.1520μg·μL-1,with the r from 0.9992 to 0.9997.Their recoveries were 98.0%,97.9%,97.4%,Respectively.The results indicated that the contents of synephrine and flavonoids were much differet between the species of different sources. With the fruits of citrus species becoming more mature, the contets of synephrine and flavonoids got lower. Fructus Citri Sarcodactylis had no synephrine and flavonoids.The studies provided testing witness and Sciencial Methods.