Obeserving The Distribution Of Intestitial Cells Of Cajal In The Colone With Hirschsprung's Disease And Allied Hirschsprung's Disease

ObjectiveThe aim of this study was to examine the distrubution of Interstitial Cells of Cajal (ICCs) in the colon of patients with Hirschsprung's Disease and with Allied Hirschsprung's Disease,and to find out the role of ICCs in the pathogenesis of Hirschsprung's disease (HD) and Allied Hirschsprung's Disease (AHD) .Method20 patients with HD,20 with AHD and 16 controls were studied. ICCs were indentified with a monoclonal antibody to c-kit(CD117) by immunochistrychemistry method. The area occupied by ICCs was calculated with ImagePro-Plus analysis software. The result was ananlised by SPSS12.0 software.ResultsICCs were abundant and locatied in the myenteric plexus(MP),the circle muscle(CM) and longest muscle(LM).In HD aganglionic colone , ICCs were very scarce, but in the proximal part ,ICCs were abuduant.The siginificiant diference was between the proximal part and the distal part (P<0.001).But the difference between the proximal colone of HD and control was not obviously (P >0.1). In AHD bowel ,ICCs cells were weaker and mainly locatied in MP in the distal clone .The distribution of ICCs in AHD distal colon was diference(P<0.001). In the distal colon,there were significiant diference in the three groups;In the proxima part ,the diference was no clear(P >0.1)ConclusionThis study reveals the relation between the abnormal distribution of ICCs and the pathogenesis of HD,AHD. It is suspected the lack or reduction of ICCs would be responsible for the gastrointestinal motility dysfunction, which is an importent factor in the pathogenesis of AHD and HD. The difference of ICC may be relatied with their different signs and symptoms.