Comparison Of Vascular Endothelial Growth Factor C And D Expression Between Primaryand Metastatic Ovrian Cancer

Objective: Ovarian cancer, originated from deep pelric difficult to detail, lack early symptoms, too late when found so it's 5-year survival rate is low, and seriously threaten wemen'life. Apart from direct spread and peritoneal seeding, lymph road is a main way epithelial origin ovarian metastasis.With progress in molecular biology, VEGF becaming a research hot. Previous studies were focused on the tumor'hematogenous route, angiogenesis, antiangiogenesis treatment and had made some progress. Now, more attentions are added to the role VEGF-C and VEGF-D act in lymphatic generation and lymph metastasis. VEGF-C and VEGF-D bind VEGFR-2 induce angiogenesis; VEGFR-3 bind VEGF-C and VEGF-D ligand regulate lymphatic endothelium chemokines, cell proliferation, differentiation, lymphatic hyperplasia, thus lead to disseminated tumor cells and lymph node metastasis. Although with deeper study on VEGF-C and VEGF-D more attention have been devoted to VEGF-C, VEGF-D and epithelial ovarian cancer, those research mainly subjected on the primary site, metastasis site was rarely rerorted. Tumor gene mutations are constant ,gene expression products changes are constant , tumor primary and metastasis charactors are changing, so it's necessary for us to study on primary site and metastasis site; conclusions VEGF expression in ovarian primary and metastasis site mixed at home and abroad; and there has no study on lymphatic generationg factor VEGF-C,VEGF-D expression in ovarian primary and metastasis site same time. This study detecte VEGF-C and VEGF-D expression in advanced ovarian epithelial primary and omental metastasis,discuss VEGF-C and VEGF-D expression difference between ovarian cancer primary and metastatic lesions and their relation to ovarian cancer occurrence development and prognosis; we also compare them according to pathological types , pathologic classification and age;make deeper study on mechanisms of tumor metastasis, provide a theoretical basis for clinical treatment and improve the prognosis.Method: 48 patients are selected from gynecological surgery specimens of advanced epithelial ovarian cancer with omental transfer in Feng-Tian hospital attached to ShenYang medical college, The fifth people'hospital of Shen Yang and zhongliu hospital liaoning between January 2001 to May 2006 who clinical stageâ…¢-â…£, no preoperative radiotherapy, untreatment of anticancer drug. 25 patients are serous cystadenocarcinoma, 5 patients are mucinous cystadenocarcinoma, 4 patients are ovarian endometrioid carcinoma, 13 patients are clear Cell Carcinoma, 1 patient is undifferentiated carcinoma; well differentiated 34 cases, moderate differentiation 10 cases, low differentiation 4cases; more than 50-year-old 43 cases, less than or equal to 50-year-old 5 cases. Using immunohisto- chemistry of streptomycin avidin Biotin peroxidase (SABC) detect VEGF-C,VEGF-D expression of different pathological type and stage in advanced epithelial ovarian cancer with omental transfer primary site and omental metastasis site, analysis of whether there differences between VEGF-C, VEGF-D in primary and metastasis; VEGF-C and VEGF-D correlation and the relation between pathological type, age and classing.Results: High expression rate of VEGF-C in advanced epithelial ovarian cancer primary site is 31.3%, metastasis site 45.8%; High expression rate of VEGF-D in advanced epithelial ovarian cancer primary site is 8.33%,metastasis site 12.5%.There is no Significant differenc VEGF-C,VEGF-D expression between advanced epithelial ovarian cancer primary site and metastasis site.There is positive correlation between VEGF-C and VEGF-D in advanced epithelial ovarian cancer primary site and metastasis site. No significant difference between pathological type (P>0.05), Significant difference between pathological classing (P<0.05). No significant difference between (P> 0.05) ages.Conclusion: 1.There is no significant difference VEGF-C, VEGF-D expression between advanced epithelial ovarian cancer primary site and metastasis site. 2. There is positive correlation between VEGF-C and VEGF-D in advanced epithelial ovarian cancer primary site and metastasis site, VEGF-C stronger expresse than VEGF-D. 3. VEGF-C,VEGF-D expression between advanced epithelial ovarian cancer primary site and metastasis site are irrelevant with pathological type and age but positive correlation with pathological classing. 4. Specific lymphangiogenic factors VEGF-C VEGF-D may be a new ideal target for ovarian cancer growth and metastasis.