Differential Proteome Of Cerebral Ganglion Including Neural Connective Induced With Both Saponins And Acrylamide In Aplysia

Aplysia is a kind of mollusk animal belonging to gastropod class, its central nervous system (CNS) is one of the classical models for molecular neuroscience research. The research on function proteins and peptides in Aplysia CNS is helpful to elucidate the activity pattern and regulation mechanism of CNS in higher animals including human, such as formation of memory, neuronal modulation and the pathology of CNS disease etc.. Panax notoginseng(PN) is one of the traditional Chinese herbs, its main ingredients are saponins, which have protective effect on nervous system, such as nootropic and delay of senility.TLC, HPLC, MALDI-TOF-MS were used to analyse the composition of Extract of Panax notoginseng (PNE). Ginsenoside Rb1, Rg1, Re and notoginsenoside R1 accounting for above 80% of total saponins were the main ingredients of Panax notoginseng.The CNS in Notarcus leachii cirrosus Stimpson(NLCS) was chosen as the experiment material. Proteomic techniques were employed to preliminary identify the differentially expressed proteins such as Actin, 3-hydroxyacyl-CoA dehydrogenase, ABC transporter and methyltransferase induced with PNE. After R1 inducement, apoptotic suppressor protein, acyl-CoA dehydrogenase, 26S proteasome and methyltransferase differentially expressed. These proteins were further subcellular located for the purpose of getting detail information. Through analogy analysis, we suggest that these differential proteins are connected with both learning, memory and neurodisease development. These work plays much important roles in revealing the action mechanism of PNE and R1, could provide a scientific basis and novel way for neurodisease prevention and therapy in future.Acrylamide(ACR) is a common neurotoxic agent, it leads to nervous system injury directly. Saponins in Panax notoginseng have the function of alleviating nerve injury. The CNS in Aplysia Juliana(AJ) was used as research material. The experimental results showed that there were 24 differential proteins in cerebral ganglion(CG) of AJ treated with ACR. But after PNE recovery processing, most of differential proteins were eliminated because of its antagonistic effect. So the expression level of these proteins was close to control group, while the effects of PNE+ACR and ACR+PNE were not totally the same. After database search, there were three proteins related to nervous system injury, such as heat shock protein 20, short chain dehydrogenase and Triose-phosphate isomerase. This work was helpful to eluminate the toxic mechanism of ACR during the metabolic process in CG, study the protective role of PNE against ACR-induced neurotoxicity. The results have guiding significance for practical application.