The Study Of Ligusticum Chuanxiong And Its Main Components And Ground Squirrel During Hibernation Anti-muscle Atrophy Of Mechanisms

Objective:1. To study the effects and their mechanism of Ligusticum chuanxiong and its two components (Sodium Ferulate and Ligustrazine as main efficacy components in Ligusticum chuanxiong for invigorating blood circulation) on muscle atrophy in a hind limb unloaded rat model.2. To study the ground squirrel's possible mechanisms of anti-muscle atrophy during hibernation.Methods:1. The tail-suspended rats were subjected to a 14-days disuse, immunohistochemic, hemorheology property and muscle fiber ultrastructure in/out muscle spindle were used to study the effects of medicines on soleus muscle.2. Blood viscometer and Electron microscope were used to investigate the ground squirrel's hemorheology property and muscle fiber ultrastructure in/out muscle spindle during hibernation.Results:1. Compared with HLU+W, 1) the CSA of soleus type I fibers in HLU+SfH and HLU+TmpH increased by 37.3% and 39.4% respectively (P<0.05); 2) expression level of MHCII were inhibited in all treatment groups (P<0.01); 3) expression of MHC in nuclear bag 2 fiber were altered from positive to negative; 4) the blood viscosity in low shear rates decreased obviously (P<0.001), even near to CON's; 5) and show the inhibited effect on change of muscle fiber ultrastructure in/out muscle spindle.2. 1) Compare with Aut-A, whole blood viscosity increase during hibernation and decrease after hibernation. 2) Hemorheology property have no significant change after hindlimb-unloading. 3) Muscle fiber ultrastructure in/out muscle spindle have no significant change during hibernation, and after hibernation it worse than H-m. Conclusion:1. Ligusticum chuanxiong and its two main efficacy components (Sodium Ferulate and Ligustrazine) can prevent soleus atrophy induced by disuse, and Sodium Ferulate and Ligustrazine in high dose showed most efficacy.2. Hemorheology property and muscle fiber ultrastructure in/out muscle spindle have relationship with physiological property of ground squirrel, this may be one of the reason that anti-muscle atrophy during hibernation.