| Taxol is one kind of natural anticancer medicine. USA chemist Wall and Wani get first from the Pacific Ocean yewtree and name for taxol and announcing whose chemistry structure in 1971. USA and a European scientist reveal out anticancer effect later consecutively, especially cancer, the peculiar curative effect to breast cancer, the ovary. As the anticancer effect mechanism is unique , active, taxol is accepted as the broad fair amount of confidence in the world already by the nowadays , the strongest anticancer activity medicine , its coming out are that the nineties international mounts anticarcinogen which is one of three big accomplishment by the reputation. But be similar to a component Diterpenes of unique structure, taxol is difficult to dissolve in water and a lot of medical menstruum. Because it was not peculiarity cell toxicity, the organ often also brings about grave damage to regular organization while being used to cure a tumour for killing a cancer cell. Exactly being that pure water-solubility of taxol is bad, big amounts with the medicament and cell toxicity heavily what is being restricted in going ahead clinically. Therefore, anticancer medicine of new model taxol becomes the focus that the medicine field studies on in recent year.Breast cancer is one of the most common cancer of female, accounts for 7% ~ 10%of the whole body cancer .what has highly morbidity and characteristic of metastasis rate , invading, but course of disease makes slow progress rather, the natural life cycle is long. Morbidity of breast cancer is increasing the coming 20 years in china, already becomes the healthy common serious harm for the tumour. Many datas indicates in 25% - 30%' of the breast cancer patient what exists amplification and over expressing for proto-oncogene HER2/neu, the survival rate is low for the breast cancer clinical patient, the lymph node transfer easily and chemotherapy catabasis shortens, It is drug fast to tamoxifen and cell toxicity chemotherapy medicine, but it is kind to big dosages nthracene nucleus and Japanese yew branchlet and leaf. proto-oncogene HER2/neu is one of important breast cancer molecular biology mark thing , both. the prognosis to breast cancer and the curing extremely important value. In recent years, openeding up a new field with tumour molecular biology studying deep every year, the tumour targets for curing and treating good peculiarity that the relatively lower poisonous side effect as breast cancer.The molecule target is turning up what is applied to the clinic broad , what has brought the gradually important effect into play in the feasible target being cured to the synthesis treating in the tumour. The antibody mediated target cures in the tumour ,the antibody is a fine medicine target carrier. Antibody with tumour conjugate is being to make use of antibody to tumour cell surface antigen choice peculiarity , anticancer medicine carrying out the driving target studying to design , improving the anticancer medicine selectivity to tumour cell , the toxicity reducing to regular organ. By the medicine molecule e peculiar function group such as: hydroxyl , Sulfhydryl , amidocyanogen etc., making the medicine and antibody to connect what is conjugate .what avoided the medicine pernicious effects to other regular organization, the field brings selectivity into play curing an effect.The main of the paper is used strengthen taxol water-solubility that is improving taxol to play breast cancer's curing effect, The purpose lessening taxol poisonous for not target side effect to the organ , what is the antibody target. Choosing taxol activity Corp. 2'- OH and setting about to modify. Adopt activation ester method , taxol is reacting with succinic anhydride to form manufacture 2'- succinic Itaxol reaction activity transform OH to COOH, 2'- succinic Itaxol and N-hydroxyl- succimide(NHS) IS crosslinking condensation , We get taxol activation ester and couple HER2iwith it. The last we get taxol- HER2 conjugate. The taxol couple with antibody has certain water-solubility , After Sephadex column which original activity is 56% or so by enzyme linked immunosorbent assay(ELISA assay) ;detecting cytostasis of conjugate to breast cancer MCF-7 type tumour by Mono-nuclear cell direc cytotoxicity assay(MTT reduction assay),what is controlled by 1.4 times of identical taxol restraint rates; low cytometry detectapoptosis cell rate the conjugate appears obvious deuto- apoptosis diploid peak what promotes apoptosis effect better than using taxil only. Detecting DNA content by agarose gel electrophoresis and cell apoptosis protein by Western-blot, is ladderlike characteristic strip belt to DNA and the content of the protein confirm the result HER-2/neu monoclonal antibody with taxol conjugateis the fact that improve taxol medicine target to kill breast cancer cell, what restrain higher tumour cell to grow and reduce dosages with poisonous organ side effect. For themethod in preparation, provides the reference and the basis for conjugate ; As introducing infrared spectra , the nuclear magnetic resonance spectrum , have perfected the appraisal activated-taxol .Detecting the activity by MTT assay, FCM assay AGE andwest-blot to have enriched the detecting method resisting the tumour effect conjugate; It is provided for low dosages, low toxicity ,high curative effect of clinical practice ,what has the certain push effect for taxolal extensive useing.
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