Study On Relations Between Differential Gene Expression And Polymophrism Of HtrA2 And Male Infertility

Infertility relates to marriage and family.As it is a healthy problem of the world reproduction.the reason of Infertility relates to men and women both sides.The reason from female calls dysgenesia.We gradually research thorough and systemic for male infertility.From the originally environmental factor to human factor.Protein expressing and gene polymorphism.So we can know specify and more complicate.In cell genetics and molecular biology spermiogenesis is developed fast and it indicates vividly with genetic factor.As chromosome abnormality and male infertility.Gene polymorphism male infertility.Y chromosome microdeletion and male infertility.Athough the male infertility at inheritance basis is obvious.People know gradually the genetic mutation relating to a series of process such as spermiogenesis.Sperm maturity and transportation besides chromosomal anomaly.The other calls male infertility from the male.Disorder of apoptosis control could be one is important factor for male infertility.By restraining IAPs and activating caspases and serine protease path urges apoptosising.Serine protease HtrA2 being released under apoptosis signal guidance from mitochondria entering kytoplasm.Therefore.Analyzing expressing of apoptosisfarom in male infertility patient forHtrA2 by studying spermatoon apoptosis.lt is used to know development mechanism of the male infertility.At present it already demonstrates HtrA2 what can pass the adjusting mitochondria in vivo or the cytoplasm controls a cell's destiny.But we still do not understand specific mechanism what has an effect in the cell.It is very important HtrA2 repairing will be a role of function,which factors had triggered this process? With HtrA2 other members: HtrA3 and HtrA4 and more substrates are founded in the HtrA family.We know closely apoptosis mechanism to HtrA2. HtrA2 belongs to the family of proteinase and it expresses commol in the tissue. localization of genes is the human chromosome 2p12.HtrA2 precursor is 50kD protein and mature HtrA2 is 40KD protein.Active HtrA2 exists trimeric form.HtrA2 is constructed of three majoraminoterminal Reaper base that it can be binding to X-linked Inhibitor of apoptosis Protcir and take an effect of apoptosis.The centre serine protease function area;C end,PDZ structure region is known by PSD-95,DIg and ZO-1 as containing fundamental fundamental protein.The main function is adjusting the HtrA2 serine protease activity and PDZ structure region.While blinding to rod-shape structure of catalytic domain to prevent reacting substrate with active protease zone. And the zone is turn to play arrive at important role to HtrA2 what takes an effect at the substrate.The zone is HtrA2 what is an effect at eliminating the protein Infolding error in the mitochondria.Likewise,HtrA2 own a parenzyme catalyzing structure region and the mitochondria structural domain.Inducing by apoptosis signaI.HtrA2 is a mitochondria of coded nuclear gene as is serine protease and have excessively a promoting apoptosis function.Especially transform to ripe molecule format with releasing to the cytoplasm from mitochondria.Mature HtrA2 promote apoptosis by being dependent on caspase and being independent on caspase the former take an effect on the union both Napical unique structure and BIR of IAPs.Then.Relieveing the IAPs restraint to caspase.The latter is related to HtrA2 protease are active.Highly expressing of HtrA2 will increase the trend to apoptosis in sperm.It may be to reduce the sperm survival rate and to affect the male sex reproduction mechanism.Then.much code gene state nature and dysgenesis correlativity by studying the HtrA2 codeing gene.We can go a step further for researching the relationship both HtrA2 code gene polymorphism and male infertility.At present there is no result for serine protease with male infertility.But as promoting apoptosis protein,it is important connection to male infertility with the document in the internal and overseas.In sperm serine protease is highly expressing obvious than the normal group,what explains the serine protease promotes spermatoon apoptosis.The experiment carefully studies the genetic mutation of serine protease in the whole coding gene.The relationship between gene mutations with coding gene.We get into the mechanism of the male infertility with the serine protease. It will provide a basis for male infertility.Objective:Researching mutational site for the the whole code gene of HtrA2 in different sperm concentration is related to male infertility.It is providing scientific basis of male infertility's diagnose and treat.Methods:This research collected semen in clinic first.Then.Useing Trizol reagent to extract total nuclear protein and genomic DNA of sperm,detected the content of HtrA2 in sperm nucleus by Western Blotting technology.Through the detection by SDS-PAGE separation and illunoblotting,the comparison of normal fertile group and the infertile group showed that the expression content of HtrA2 in infertile patients increase obviously.We can draw a conclusion that there is some relationship between the abnormal expression content of HtrA2 and male infertility.To extract genomic DNA from sperm nucleus,to get HtrA2 genome whole sequence by retrieval GenBank database, unique primers were designed by software Primer3.0 and Oligo6.0 on the line HtrA2 includes 8 exons sequences.The detecting making use of PCR-SSCP law seeks having the abrupt change place to measure order after analysing or not in rough detecting mutational site roughly by PCR-SSCP and sequencing analyze.Results:The expression content of HtrA2 in infertility patients with oligospermatism and lepto-sperm has increased.The content in sperm count normal group has not changed.There is no mutational site founded in the whole code zone for HtrA2 in male infertility patients.Conclusion:The abnormality of HtrA2 has some effect on the sperm production, may also an important factor lead to male infertility.It is not correlated with male infertility in no mutational site of HtrA2 coding gene.