Study On Semen Routine Analysis,Sperm DNA Damage And Sperm Apoptosis In Male Infertility Patients With Kidney-yang Deficiency And Kidney-yin Deficiency

Objective:Through the detection of 89 cases of semen routine,sperm apoptosis rate and sperm DNA fragmentation index,to explore the kidney yang deficiency,kidney yin deficiency male infertility patients and other infertile patients between the semen routine,sperm apoptosis rate,Sperm DNA fragmentation index and the correlation between sperm apoptosis rate and sperm DNA fragmentation index in male infertility patients.Method:Kidney yin deficiency group(N = 30),kidney yin deficiency group(n =30)and control group(n = 29)were used to collect semen samples from male infertility patients,including computer-assisted semen analysis(n = 30)(FCM)and acridine orange(AO)were used to detect the sperm apoptosis and sperm DNA of the spermatozoa.The flow cytometry(FCM)Fragmentation index(sperm DNA Fragmentation Index,DFI).Statistical analysis of the collected data was carried out using SPSS20.0 statistical software.The measured data were expressed as the mean deviation(X ± S).Comparison between groups using two independent samples of the t test.Sperm apoptosis rate and DFI correlation were analyzed by Pearson correlation analysis based on the normal distribution or skewness of the data.P<0.05 that the difference was statistically significant,P<0.01 for the difference was statistically significant.Results:1.Compared with the control group,there was no significant difference between the two groups(P>0.05).There was no significant difference between the kidney yang deficiency group and the kidney group Compared with P>0.05(P =0.404),no significant difference.2.Compared with the control group,there was no significant difference between the control group and the control group(P>0.05).There was no significant difference between the kidney and the yin deficiency group and the kidney yang deficiency group.The percentage of sperm motility in the a group was lower than that in the control group(P<0.05).The percentage of sperm motility was lower than that of the control group(P<0.05)(P<0.05).There was no significant difference between the control group and the control group(P>0.05).The percentage of sperm motility,the percentage of sperm activity and the sperm motility were lower in the kidney-yang deficiency group than in the control group(A + b grade sperm percentage P = 0.050,spermatozoa)were significantly lower than those in the control group(P<0.01)Activity rate P = 0.047).3.The apoptotic rate of spermatozoa in kidney-yin deficiency group was higher than that in control group,P<0.05(P = 0.028)was statistically significant,and DNA fragmentation index was higher than that of control group,P<0.01(P = 0.000)The spermatic apoptotic rate and sperm DFI were significantly higher in the kidney-yang deficiency group than in the control group(P<0.01(sperm apoptosis rate P = 0.010,sperm DFI index P =0.000).4.The sperm apoptosis rate and DFI results of all the subjects were analyzed by Spearman correlation analysis,and the correlation coefficient was 0.285,P = 0.007,which was statistically significant.Conclusion:1.The sperm motility and sperm viability were significantly lower in the patients with infertility than that of other male infertility patients.The degree of DNA fragmentation and sperm apoptosis were higher than those of other male infertility patients.Kidney yang deficiency,kidney yin deficiency male infertility patients and other infertile patients compared with sperm motility and fertility lower.2.There was a significant positive correlation between sperm DNA fragmentation rate and sperm apoptosis rate in male infertility patients,suggesting that sperm DNA damage may be related to sperm cell apoptosis.