Experimental Study On The Pharmacodynamics Of Chubitong Capsule In Treatment Of Rheumatoid Arthritis

Chubitong capsule is developed by Professor Gao Pengxiang's owned experince who is the famous doctor of Ji Lin Province.The prescription is including Aconite Root,CORTEX ILLICII,Acanthopanax Trifoliate Root-bark,Rhizoma Drynariae,Himalayan Teasel Root,Scurrula parasitica,Icraiin,Twotooth Achyranthes Root,Zaocysdhumnades,Safflower,Trilobate Wedelia,Ephedra Herb,Cassia Twig,Licorice root etc.14 kinds of Chinese traditional medicine. It mainly treats on the Cold and damp arthralgia which is indicated in clinical manifestation as muscle physique arthralgia ache,anaesthesia,or arthrocele ardor.Below is the pharmacologic action of studying of Chubitong capsuleObjective:This study is to study effectiveness of Chubitong ca psule through the experimental studies on experimental arth ritis,immune function and analgesic effect,etc.,then pro viding possible theorem and experimental foundation for the capsule' s clinic trial and application.Method:The pharmacodynamic action experiments are including 1 How does Chubitong capsule depressant effect on adjuvantinduced arthritis of rats which is to inject 0.1ml of Freun d' scomplete adjuvant on right volar hypo of rats,to dose capsule one hour later after inflammation,at the same time to measure the varicose level—the adjuvant-induced arthri tis varicose level is defined to equal the different value before and after dosing,and also to observe the situation such as auricular erythema and caudal nodus etc..2 How does Chubitong capsule effect on SN-263 cryptopodia of rats,which is to inject 0.1ml of 1%SN-263 in right rear foot aponeurosis palmaris of rats,and then using the narrowband to measure the difference value between right-left gambrel and circumference of foot vola,that is cryptopodia level.3 How does Chubitong capsule effect on SN-263 cryptopodia of mouse,which is to inject 0.03ml of 1%SN-263 in right rear foot aponeurosis palmaris of mouse,and 6h later after hypersusceptibility,then cutting off the two postpedes and weight them respectively,the different value is the cryptopodia level of mouse.4 How does Chubitong capsule effect on cotton pellet granuloma of rats,which is to put the rat to death,peel off the granuloma and dry it into hot oven under 60℃,then weight it 5 How does Chubitong capsule effect on abdominal cavity blood capillary permeability of mouse? Measure the photometric value of eluant in abdominal cavityThe effect on immune function:1 How does Chubitong capsule effect on delayed hypersensitivity caused by 2,4-(DNCB) of mouse.On the 10th day,comparing with 0.25%dinitrochlorobenzene,after 38h,cut off two feet below the anklebone and measure those on the electronic balance respectively,take the different value as the DTH target.2 How does Chubitong capsule effect on endothelial system dysphagocytosis of mouse,which is to do carbon particles clearance experiments? After injection prepared Chinese ink of Yidege in the trail of mouse,take 40μl blood into 4ml distilled water test tube from orbital vein in 2m and 15m later,then measure the 0D and calculate the rate of carbon particles clearance in blood in minute,meanwhile taking and weighting the liver and spleen to verify K value.Abirritation:1 How does Chubitong capsule effect on writhing response of mouse caused by acetic acid,which is to inject 0.8%acetic acid liquid as 0.1ml/10g according to weight? Observe the times and time of writhing response in ten minutes 2 How does Chubitong capsule effect on hot plate pain threshold of mouse? Measure the threshold value for each group after 1 hour and 2 hour laterResult:The experiment result indicates that:Through drenching different doses Chubitong capsule,it has obviously depressant effect on the foot varicose of r at which is caused by injection Freund's complete adjuvan t,what is Chubitong capsule has obviously depressant ef fect on complete adjuvant arthritis.And also it can contro l the increasing of abdominal cavity capillary permeability of mouse,and also can control cryptopodia of rat and mou se caused by SN-263,at the same time control the cotton pellet granuloma of rat.It can depress delayed hypersensi tivity caused by 2,4-(DNCB) of mouse.Besides,it can depress the pain obviously if dosing once timeConclusion:Chubitong capsule has obviously effective the antiinflammat ory,immunodepressive and easing pain,which provides some pharmacological basis on curing arthritis deformans clinica lly...