| Radix Rehmanniae is the fresh or dry root tube of the Scrophulariaceae plant Rehmannia glutinosa Libosch.,which is one of the four famous herbs produced in Henan HuaiQing.The history of its pharmaceutical use is long in time.It is one of medicinal substances frequently used for invigoration.Radix Rehmanniae is usually separated to the fresh Radix Rehmanniae,the dry Radix Rehmanniae and Radix Rehmanniae Praeparata on the basis of pharmacodynamic difference;the efficacy of the fresh Radix Rehmanniae that can remove heat to promote salivation and stop bleeding is better than that of the dry one,however,the efficacy of the Radix Rehmanniae Praeparata is replenishing vital essence to tonify kidney. Iridoid glycosides is the most chemical composition in Rehmannia glutinosa Libosch.,their heteropolarity is usually great and they can dissolve in water very easily.If heated,they are easily changed to other components.So the distinctive chemical composition in the fresh Radix Rehmanniae makes its efficacy unique. But the fresh Radix Rehmanniae is not easy to keep,its most active constituents could be easily destroyed by incorrect storing method,which constraints its macket circulation and clinical application.We can resolve these problems by preparing the fresh Radix Rehmanniae extraction whose quality is stable and controllable.In addition,We need retain the most active and fingerprint constituents in the extraction to ensure clinical application and the development of new drugs.As one of the four famous herbs in Henan province,Rehmannia glutinosa Libosch.has affluent resoures and adequate quality.The fresh Radix Rehmanniae extraction is to increase the technical added value of the medicinal substances and procure good social effects and economic returns.In the first chapter,we firstly summarize the study on the chemical composition and assay method of the iridoid to provide reference for the study of these components in Rehmannia glutinosa Libosch.;Secondly,we summarize the study on the chemical composition,purification technology,assay method and fingerprint of Rehmannia glutinosa Libosch.for deeper study and development.In the second chapter,we explore the optimum extracting conditions for Rehmannia glutinosa Libosch.The orthogonal design was taken for the option,the contents of catalpol,Rehmannioside D and leonuride were determined by RP-HPLC.The optimum extracting conditions for Rehmannia glutinosa Libosch. were as follows:The smashed Rehmannia glutinosa Libosch.were extracted for three times,adding 6 times amount of 20EtOH every time.In the third chapter,we investigate the technological parameters of the purification process of iridoid glycosides in Rehmannia glutinosa Libosch.with macroreticular resins.The objective components were detected by HPLC or TLC, the static capacity absorption and dynamic state elution ratio were observed,nine types macroreticular resins were evaluated.We find that SP-700 type macroreticular resin showed better comprehensive absorption property.It can be used to purify the iridoid glycosides of Rehmannia glutinosa Libosch.SP-700 macroreticular resin can raise the content of catalpol in extractive from 6%to 18%, Rehmannioside D from 0.3%to 1.7%,leonuride from 0.2%to 2.6%.In the fourth chapter,we establish quality standard for the extractive of Rehmannia ghttinosa Libosch.The catalpol,Rehmannioside D and leonuride in the extractive were identified by TLC and determined by HPLC.From the results, we find the identified characteristics of chromatography were distinct and highly specific.The calibration curve of catalpol showed a good linearity within the range of 0.32-1.60μg,γ=0.9996.The average recovery and RSD were 99.74%and 2.55% (n=6) respectively.The calibration curve of Rehmannioside D showed a good linearity within the range of 0.406-2.03μg,γ=0.9998.The average recovery and RSD were 96.14%and 1.36%(n=6) respectively.The calibration curve of leonuride showed a good linearity within the range of 0.8-4.0μg,γ=0.9999.The average recovery and RSD were 100.10%and 2.73%(n=6) respectively.The established method is simple,feasible and repeatable.It can be used for the quality control of the extractive of Rehmannia glutinosa Libosch.
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