Endostatar In The Treatment Of The Transplantable Model Of Human Nasopharyngeal Carcinoma In Nude Mice

ObjectiveTo study the effect and mechanism of Endostar on the growth and the vascular product of nasopharyngeal carcinoma in the nude mice.MethodsEstablished the nide mice model of transplantation tumor of CNE2 nasopharyngeal carcinoma.Divided into the low,middle and highe dosage groups,blank control group.Compared the inhibithon effect of tumor among the groups.Tumor tissues were obtained and used for immunohistochemical staining for CD34,which is the indicator of microvessel density,and VEGF,after giving 21 days medicine.CNE2 cell apoptosis in nude mice after giving highe dose were detected by transmission electron microscope.The cell apoptosis rates and cell cycle was detected by cell flow cytometry.Results(1) Endostar could inhibit the growth of transplantation tumor obviously,the volume of tumor in each of low,middle,highe dosage groups was much less than blank contral group respectively after giving 21 days medicine.(P＜0.001),the inhibit rate of big dosage group was 42.8%.(2)MVD in each of low,middle and hige dosage groups was much lower than the one of blank contral group respectively.(P＜0.05, P＜0.01,P＜0.001). (3)Expression of VEGF of each of Endostar groups were lower than the one of blank group respectinely.There has obvious difference between each of Endostar groups and blank group on expression of VEGF(P＜0.01).(4) CNE2 cells apoptosis was observed in nude mice by transmission electron microscope after giving highe dose. (5) Flow cytometry analysis revealed that treating CNE2 cells with increasing quantities of Endostar increased the percentage of cells in the G0/G1 phase.ConclusionsThese studies showed that Endostar could significantly restrain the development of nasopharyngeal implanted tumors with time-dose-dependent in nude mice.Endostar has obvious antiangiogenesis of nasopharyngeal carcinoma,its mechanism was probably related to the inhibition of VEGF expression.Endostar had a role in inhibiting the proliferation of CNE2 cells in nude mice in a dose dependent.The antiproliferation property of Endostar in cultured human nasopharyngeal carcinoma CNE2 relates to its ability to arrest G0/G1.