| Objective: To investigate the cellular compatibility of silk fibroin/hydroxyapatite (SF/HA) and its efficacy in repairing contained bone defects, and to explore the possibility of its clinical application.Methods: SF/HA was co-cultured with Adipose-derived stromal cells(ADSCs)to repair bone defects.36 rabbits were randomly divided into 3 groups. Each group had 12 rabbits. The bone defects(4.5mm×4.5mm×10mm) in the condylus lateralis femoris of rabbits were implanted with ADSCs-SF/HA complex(group A),with simple SF/HA(group B) or left untreated(group C).Result: Normal adherence, proliferation and growth of ADSCs on the SF/HA could be seen. The examination of X-ray and histology showed that the bone defects were repaired entirely in group A and partly in group B. The quantity of the newly formed bone in group A was more than that in group B (P <0.05). Repair showed no effect in group C.Conclusion: SF/HA has good cellular compatibility, and can successfully repair contained bone defects of a rabbit femur. It can be used as the ideal alternative biomaterial for bone tissue engineering.
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