| Objective:Observe the influence of the pushing-palpating channel-moving apparatus on the index of gallbladder volume and shrinkage of patients after the mini-invasive endoscopic stone extraction.By the treatment of pushing-palpating channel-moving apparatus on rabbit,we observe the influence of its Gallbladder size and the expression of CCK-AR mRNA in the gallbladder smooth muscle.From the kinetic point of view we investigate the mechanism of the pushing-palpating channel-moving apparatus,provide a more rational approach and scientific basis for clinical application of this apparatus.Methods:This research is divided into clinical research and animal experiments. The total 73 research objects of the clinical research are the patients of the mini-invasive endoscopic stone extraction treatment in TCM Hospital of Shenzhen City from 2006.10 to 2008.5,use randomized single-blind method, select 73 random number continuously in random number table.In accordance with the order of patients admitted to hospital,each corresponding to a random number,odd-numbered into the treatment group,that is,38 cases of treatment group;Even included in the control group,that is,35 cases of conventional treatment.We treat the treatment group with the pushing-palpating channel-moving apparatus one month after stone extraction,they eat the fat meals at 8:30am that day 12 hours after an empty stomach,and do treatment after 15 minutes,Take the head low-foot high(about 15°) left lying,use the electrode plate treatment,an electrode placed on the right side of DanShu, another Placed in the Gallbladder point,select the I Output frequency.Each treatment last for 30 minutes,one times a month,the total course of treatment is one year.Observe the differences of the Gallbladder wall thickness and shrinkage function between treatment group and control group,then use SPSS10.0 to analyze the result.We select 28 New Zealand rabbits for animal experiment(provided by Guangzhou University of Traditional Chinese Medicine Laboratory Animal Center,I-class),the weight is 2.0-2.5kg of either sex, randomly divided into 3 groups,use the same feeding patterns.The blank group do not use the pushing-palpating channel-moving apparatus treatment. Experiment group 1 use the pushing-palpating channel-moving apparatus treatment once 12 hours after Fasting,extract the gallbladder wall. Experiment group 2 use the pushing-palpating channel-moving apparatus treatment once a day only during Fasting,lasting 7 days,then extract the gallbladder wall.The treatment method is fixing animals in supine position, select the right DanShu and QiMen,use I Output frequency.We use pushing handle and Plate treatment,20min for each treatment,Control the stimulus intensity between 5—15.measure the difference of its Gallbladder size and the expression of CCK-AR mRNA in the gallbladder smooth muscle of Rabbits in each group,then use SPSS10.0 to analyze the result.Results:1.After two sets of clinical data t-test,P>0.05.There were no significant differences,they are comparable.2.Evaluation of clinical efficacy:the compare result of Gallbladder wall thickness is the control group > the treatment group;the compare result of gallbladder shrinkage function is the control group < the treatment group. Gallstone recurrence of the situation is:1 cases of the control group, treatment group,no recurrence.Description is that the treatment group is better than the control group.3.The results of animal experiments:the compare result of gallbladder volume and the expression of CCK-AR mRNA in the gallbladder smooth muscle is that experiment group 1 and experimental group 2 both < control group.It has statistics Significance(P<0.05).Experiment group 1 and experimental group 2 have no significant difference(P>0.05).This illustrate that treatment group in these two aspects of the regulating role of indicators is better than the control group,and compared with single treatment,continuous treatment has no significant influence.Conclusion:The treatment for patients after the mini-invasive endoscopic stone extraction has the potential to reduce the recurrence of gallstone.By enhance the expression of CCK-AR mRNA in the gallbladder smooth muscle,this apparatus can strengthen gallbladder shrinkage function and increase Bile excretion to prevent gallstone recurrence.It illustrate the Scientific and feasibility of the pushing-palpating channel-moving apparatus Clinical Application. Worthy of further promotion in the clinical application. KEYWORDS pushing-palpating channeI-moving apparatus;Gallstone;Recurrence Prevention;the expression of CCK-AR mRNA in the Gallbladder wall... |
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