The Preliminary Study On Cardiac Function Recovery Using Autologous BM-MSCs Transplantation With Anti-inflammation Treatment In The Acute Period After MI

OBJECTIVES: Use autologous BM-MSCs transplantation with anti-inflammation treatment in the acute period after myocardial infarction (MI), and detect the change of level of inflammatory factors, the heart function and the morphological change of myocardium. Also the mechanism was preliminarily approached.METHODS: 25 healthy Taihu Meishan swines were randomly divided into five groups after the models of myocardial infarction were established. Group A was a control group. Group B was injected pyrrolidine dithiocarbamate (PDTC) in acute period after MI. Group C received a stem cell transplantation at the same period. Group D received both PDTC injection and stem cell transplantation in acute period after MI. Group E was another control group in which stem cell was transplanted 4 weeks after MI. In each group concentration of IL-10, TNF-αand CRP in blood serum were detected at different time points, and the heart function was measured meanwhile. Hearts histological detection was done 4 weeks after stem cell transplantation and new vessels density was also counted.RESULTS: Compared with other two groups, concentration of anti-inflammation factor IL-10 in Group D and Group B steped up ahead of schedule(p<0.05), and concentrations of TNF-αand CRP decreased obviously at the same time points. Heart functional parameters including LVDP, + dp/dtmax and - dp/dtmax were all improved significantly in Group D and Group E after stem cell transplantation(p<0.05). New vessels density in infarction junctional zone in Group D and Group E increased obviously after transplantation(p<0.05).CONCLUSIONS: 1. The expression level of inflammation factors decreased after PDTC injection in acute period after MI, which showed obvious inhibition of inflammation in infarction tissue. 2. Combination of stem cell transplantation and anti-inflammation treatment in acute period after MI could increase heart function by inhibiting the progress of ventricular reconstruction. 3. Combination of stem cell transplantation and anti-inflammation treatment in acute period after MI could improve the blood supply in the infarction junctional zone.