State Of Chromosome 3q27 And Different Subtypes Of Diffuse Large B-cell Lymphoma And Their Prognostic Relation

Objective To study the immunophenotypic and genetic features of diffused large B-cell lymphomas (DLBCLs), and their relationship to prognosis.Methods Seventy-three cases of DLBCLs with follow-up data were studied by using immunohistochemistry for CD3, CD10, CD20, Bcl-6 and MUM-1. The DLBCLs were classified into germinal center B-cell like (GCB) and non-germinal center B-cell like (non-GCB) subtypes according to Hans'algorithm. Fluorescence in-situ hybridization (FISH) for bcl-6 gene expression (located on chromosome 3q27) was performed on paraffin-embeded tissues of 54 cases.Results In the 73 cases studied, 16 cases(21.9%)belonged to GCB subtype and 57 cases(78.1%)belonged to non-GCB subtype. Breakage of 3q27 was detected in 11 of the 54 cases (20.4%), and proliferation was detected in 14 cases (25.9%). The 5 years overall survival rate of GCB subtype was significantly higher than that of non-GCB subtype (78% versus 40%, p=0.011). The bcl-6 positive cases had a better clinical outcome than that of the bcl-6 negative cases (p=0.041). Breakage of 3q27 predicts a worse overall survival.Conclusions The current study shows that the prognosis of GCB subtype of DLBCLs is better than that of non-GCB subtype. The expression of bcl-6 protein predicts a better clinical outcome, while the breakage of 3q27 predicts a worse overall survival.