Relationship Between Expression Of CA125, OPN, YKL-40 And B7-H4 And Its Prognostic Implication In Ovarian Tumor

Objective: To investigate the expression of CA125, OPN, YKL-40 and B7-H4 in ovarian tumor, and to provide a new pathological evidence for evaluating clinical treatment and prognosis.Methods: Expression of CA125, OPN and YKL-40 was detected by immunohistochemistry method in 13 normal ovarian tissues, 20 benign tumors(12 serous carcinomas, 8 mucinous carcinoma), 20 borderline tumors and 40 ovarian cancers(30 serous carcinomas, 10 mucinous carcinoma). Their relationship with age, histology type, pathology stage, cell differentiation, lymphametastasis and prognosis was analyzed.Expression of B7-H4 was detected by immunohistochemistry method in 13 normal ovarian tissues, 20 benign tumors(12 serous carcinomas, 8 mucinous carcinoma), 20 borderline tumors and 40 ovarian cancers(23 serous carcinomas, 3 endometrioid carciniomas, 6 clear cell carcinomas and 8 mucinous carcinoma). Its relationship with age, histology type, pathology stage, cell differentiation, lymphametastasis and prognosis was also analyzed.Results: The expression of CA125, OPN was significantly higher in ovarian cancer than in ovarian borderline tumor and benign tumor (P<0.05), and the expression of YKL-40, B7-H4 was significantly higher in ovarian borderline tumor and ovarian cancer than in benign tumor (P<0.05). In 40 cases of epithelial ovarian carcinoma(30 serous carcinomas, 10 mucinous carcinoma), with the higher clinical stage, the higher grade, the positive expression of CA125, OPN and YKL-40 were higher, there were significant difference(P<0.05). The positive expression of CA125, OPN and YKL-40 were also found 28/30,25/30,20/30 in serous; 5/10,4/10,3/10 in mucinous, there were higher expression in serous carcinomas than in mucinous carcinomas, there were significant difference(P<0.05). The positive expression of CA125, OPN and YKL-40 were higher in in lymde matasis than in negative lymde matasis, there were significant difference (P<0.05). The positive expression of CA125, OPN and YKL-40 were 4/6, 5/6, 4/6 in≤49 years old; 29/34, 24/34, 19/34 in >49 years old. There were not significant difference(P>0.05). In 40 ovarian cancers(23 serous carcinomas, 3 endometrioid carciniomas, 6 clear cell carcinomas and 8 mucinous carcinoma), with the higher clinical stage, the positive expression of B7-H4 was higher in every stage, there were significant difference(P<0.05). The positive expression of B7-H4 was found 21/25 in G1-G2; 15/15 in G3, there were not significant difference in the different grade(P>0.05). The positive expression of B7-H4 was also found 23/23 in serous; 3/3 in endometrioid; 6/6 in clear cell; 4/8 in mucinous. The positive expression of B7-H4 decreased significantly in mucinous carcinoma, compared with the other histological types, there were significant difference(P<0.05). The positive expression of B7-H4 was found 25/27 in negative lymde matasis; 11/13 in lymde matasis, the positive expression of B7-H4 was 5/6 in≤49 years old; 31/34 in >49 years old, in each group, there were not significant difference(P>0.05).Conclusion:1 CA125, OPN and YKL-40 are over-expressed in ovarian cancer. Their expression is closely related with clinical stage, histological grade, histotype, lymde matasis and patient prognosis, independent of age. They may reflect the progression of ovarian cancer.2 The expression of B7-H4 is correlated with the tumor histotype, clinical stage and patient prognosis, independent of age, histological grade and lymde matasis. The results suggest that the expression of B7-H4 on tumor tissue may contribute to negative regulatory immunue responses against TILs in ovarian cancer.3 CA125, OPN, YKL-40 and B7-H4 may be new targets of diagnosis and/or treatment for ovarian cancer.