Study On Extraction And Anti-oxidation Effect Of Flavonoids From Dongzao Jujube

Objective:To study the excellent extracting condition for falconoids in the Dongzao Jujube Extract(DJE) by the method of ultrasonic extracting,through monofactorial experiments and orthogonal experiments and investigate the DJE falconoids effects on the stability and oxidization in different processing and saving conditions and evaluate the DJE effects of antioxidative activity by establishing the S₁₈₀-tumor bearing mice model.Methods:1.Studied the extracting method by ultrasonic:Based on the monofactorial experiments to study the L₉(3⁴) orthogonal experiments,the excellent falconoids extracting condition could be sure through analysis of range and variance methods.2.Stability of flavones in DJE:To determine the content of total flavonoids in DJE and the cleaning capability of DHHP,comparative studied the influences in every external factor(time,illumination,power of hydrogen,metal ion,commonly used food additive and so on) for the content of falconoids and antioxidative stabilizer.3.Determination of the DJE's half lethal dose(LD₅₀):Determined the DJE's half lethal dose(LD₅₀) with Horn's method.4.Animals grouping and modeling:50 healthy Kunming mice,half male and half female,were randomly divided into five groups with 10 mice in each group.0.2mL S₁₈₀ mouse sarcoma cells suspension(concentration of 1.0×10⁷cfu/mL) was inoculated subcutaneously into the left armpit region of each tumor model mouse.Mice in the EED group and EAED group were gavaged by100mg/(kg·d) EED and100mg/(kg·d) EAED respectively.Mice in the CY control group were given 20mg/(kg·2d) CY (Cyclophosphamide injection(CY)).Mice in the CY control group,tumor control group and blank control group were gavaged 0.15mL/10g normal saline once a day.The experimental period was 10 days.5.Functional evaluation of antioxidative system:The activities of superoxide dismutase(SOD),glutathione-peroxidase(GSH-Px),the content of malanydiadehyde (MDA) in plasma and liver homogenate,hemolytic degree of RBC and single-cell gelelectrophoresis(SCGE) were measured respectively.Results:1.Every factor could affect the falconoids in DJE,the order was:extracting times＞ratio of dosage liquor＞concentration of ethanol.The excellent falconoids extracting conditions for ultrasonic extracting were as follows:The ration of dosage liquor was 1:25;the concentration of ethanol is 70%;the extracting time is 30 min.2.It could be seen that thermostability,light stability and pH limited in 5.0-8.0 were stationary in the DJE's steady experiment.There were no significant effects by the NaCl (0.025%-4.0%) or cane sugar(1%-20%) for the stability of flavones in DJE.There were little effect on DJE stability by the metal ion such as K⁺,Mg²⁺,Zn²⁺,but Cu²⁺,Fe³⁺ could cut down the stability of flavones in DJE.3.The dosage of LD₅₀ was above 5000mg/kg,which actually belongs to the non-toxic level.4.DJE had the effect of increasing the SOD activity in plasma.The SOD activities in EED and EADE groups were both significantly higher than that in the CY control group (P＜0.05) and the tumor control group(P＜0.05).And there were no significant difference between EED and EAED groups(P＞0.05).DJE had the decreasing effect on the level of MDA.The MDA level in the two DJE group were both significantly lower than that in the tumor group(P＜0.05) but both higher than that in the blank control group(P＜0.05).There were no significant difference between EED and EAED groups(P＞0.05).There was no significant difference(P＞0.05) on the every results of liver homogenate between the two DJE groups and tumor group.There was significant protection effect of oxidative damage on the blood-lympho-cytes in vitro in SCGE experimentation.Conclusion:1.The excellent falconoids extracting conditions for ultrasonic extracting were as follows:The ration of dosage liquor is 1:25;the concentration of ethanol is 70%;the extracting time is 30 min.2.DJE's flavones are stationary.3.DJE can raise the S₁₈₀-tumor bearing mice's antioxidation activity.