| Effects ofβ-carotene on the small intestine mucosal barrier and the absorption function of acute X-ray caused abdominal injury ratsAbstract Objective To investigate effects ofβ-carotene on the barrier and absorption function of acute X-ray caused abdominal injury rats.Method:40 female SD rats were randomly divided into 4 groups:Group C was the blank control group.Group R was the radiation group that accepted only X-ray radiation.Group T1 acceptedβ-carotene 5mg/ kg-d,and group T2 acceptedβ-carotene10mg/kg·d.After 14 days of continuous administration of peanut oil in groups C and R or beta-carotene(2.5ml/kg) in groups T1 and T2,the rats were radiated under X-ray of a 9 Gy dose.Then the rats were fed 0.5g/kg D-xylose orally at the 3rd day of the radiation.The 12 hours'urine was collected to test the content of D-xylose.3 days later,the rats were killed and the amount of diamine oxidase(DAO) and the level of bacterial endotoxin and TNF-αwere detected.The structure and length of the crypt-villus axis(CVA) and TNF-αof jejunum mucosa were also observed and analyzed.Resμlts Obvious damaged structures of jejunum were observed in group R,including villus adema,necrosis,and the focal exfoliation of the epithelial cell.Damage of jejunum in groups T1 and T2 were relatively minor compared to group R with less adema,necrosis,et al.CVA length of group T2 was significantly longer than those of groups R and T1(p＜0.05).Furthermore,the level of endotoxin and TNF in blood and gut mucosa were lower than those of groups R and T1(p＜0.05).The content of D-xylose in urine was higer than those in groups R and T1(p＜0.05).But there was no significant difference in amount of DAO among all the groups(p＞0.05). Conclusions Beta-carotene may decrease the X-ray radiation damage on jejunum and maintain the normal function of gut mucosa barrier damaged by X-ray radiation in rats.
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