The Effect Of Low-dose Radiation On EPO And VEGF Of Tumor In S180 Sarcoma-bearing-mice

Objective:To observe the effect of low-dose radiation on daily condition of mice and its tumor-growth;to study the effect of low-dose radiation(LDR) on hypoxia related factor EPO and VEGF of tumor in S180 sarcoma-bearing-mice;to investigate the mechanism of action of low-dose radiation.Method:Fifty-eight kunming stain male mice were implanted the S180 sarcoma cell in the left hint inguen subcutaneously as an experimental in situ animal model;grouped into the group sudo-shaming group(N) and the group LDR;12 days after implantation,the mice in group LDR were given 75mGy total-bodyγ-ray radiation.The mice in group N were sacrificed on 6h after sudo-shaming,and the mice in group LDR were sacrificed on 6h(group LDR-6h),12h(group LDR-12h),24h(group LDR-24h),48h(group LDR-48h),72h(group LDR-72h) differently, measured the tumor volume and weight,and then detected the EPO expression and VEGF expression in tumor.Results:The tumor weight of mice in groups LDR-48,72 is different statistically comparing to weight in group N;the expression of EPO is declining as time which is different statistically in group,group LDR-48h(p<0.01) and group LDR-72h(p<0.01) comparing to that in group N;the expression of VEGF is declining as time after radiation,which is different statistically in every group.Conclusion:The tumor in tumor-bearing mice had changes in 72h after low dose total radiation,and the expression of EPO and VEGF reduced.It indicated that low dose total body radiation could reduce the expression of VEGF and EPO,inhibit growing and hypoxia of tumor,improve sensitizes of radiotherapy and chemotherapy.