The Effect Of Low-dose Radiation On PCNA And EGFR Of Tumor In S180 Sarcoma-bearing-mice

Objective: To observe the effect of low-dose radiation on daily condition of mice and its tumor-inhibition effect; To study the effect of low-dose radiation (LDR) on PCNA and EGFR of tumor in S180 sarcoma-bearing-mice; to investigate further the mechanism of action of low-dose radiation.Method: Kunming stain male mice were implanted the S180 sarcoma cell in the left hint inguen subcutaneously as an experimental in situ animal model; grouped into the group sudo-shaming group(N) and the group LDR;12 days after implantation , the mice in group LDR were given 75mGy total-bodyγ-ray radiation. The mice in group N were sacrificed on 6h after sudo-shaming , and the mice in group LDR were sacrificed on 6h(group LDR-6h),12h(group LDR-12h),24h(group LDR-24h),48h(group LDR-48h),72h(group LDR-72h) differently, measured the tumor volume and weight, and then detected the PCNA expression and EGFR expression in tumor.Results: the tumor weight of mice in groups LDR is no different statistically comparing to weight in group N; the PCNA expression is depressing as time which is different statistically in group LDR-24h(p<0.05),group LDR-48h(p<0.01) and group LDR-72h(p<0.01) comparing to that in group N; the EGFR expression is declining firstly after radiation ,which is the lowest on 24h after radiation, and it is different statistically in group LDR-12h comparing to that in group N(p<0.05).Conclusion: The tumor in tumor-bearing mice had no clearly changes in 72h after low dose total radiation, and the expression of PCNA and EGFR reduced. It indicated that low dose total body radiation could inhibit the growing of tumor and reduce the expression of PCNA and EGFR in a way.