Investigation On Influence Of T-Lymphocyte Subsets And Cytokines Correlating To Th1/Th2 Cells On The Immunological Effect Of Gene Recombinant HB Vaccine

Objective To investigate the influence of T-lymphocyte subsets and cytokines correlating to Th1/Th2 cells in peripheral blood on the immunological effect of gene recombinant hepatitis B (rHB) vaccine in health adult and try to make clear the pathogenesis of non-respond to recombinant HB vaccine. It could be provided the scientific basis for researching effective immunity adjuvant and the improvement of immunity method.Methods Based on the surum level of anti-HBs in the subjects, the vaccinees were divided into hyperresponders and non-or hyporresponder groups. 52 individuals were admitted into hyperresponder group defined by an anti-HBs titer≥10 mIU/ml. The hyporresponder group was composed of 55 individuals selected with anti-HBs titer of 0-9 mIU/ml. CD3+, CD4+ and CD8+ T-lymphocyte in Peripheral blood in the two groups were investigated by flow cytoanalyze named EPICS XL model. Using ELISA assays, the OD value of IFN-γ,IL-4 cytokines correlating to Th1/Th2 were detected. The concentration of IFN-γand IL-4 in the serum was calculated and the comparative analysis was carried on.Results The percentage of CD3+, CD4+ and CD8+ T-lymphocytes in the two groups was 65.47±9.16, 33.02±5.58, 33.84±6.6 and 67.64±7.63, 25.49±6.57, 26.71±7.01 respectively, and the concentration of IFN-γand IL-4 was 14.95±8.06, 7.81±4.18 and 7.18±7.05, 2.42±1.99 respectively. There were significant differences in the percentage of CD4+ and CD8+ T-lymphocytes and the concentration of IFN-γand IL-4 between the two groups ( P﹤0.001), while no significant difference was seen in the per- centage of CD3+ T-lymphocyte between the hyperresponders and the non- or hyporresponders (P﹥0.05). The data suggested that the percentage of CD4+ and CD8+ T-lymphocytes and the concentration of IFN-γand IL-4 in the sera was correlated to vaccination response.Conclusions The results show that the quantity and the quality of CD4+ and CD8+ T-lymphocytes in peripheral blood are closely correlated to rHB vaccination response. While the quantity of CD3+ T-lymphocyte cannot influence the immunological effect of rHB vaccination. IFN-γand IL-4 cytokines correlating to Th1/Th2 cells in peripheral blood caused by a defect in HBsAg-reactive T cells or the primary HBsAg-specific T cell repertoire also may be one of the reasons and mechanisms of nonresponding to hepatitis B vaccination. The study demonstrates that IFN-γand IL-4 could be used as the adjuvant in vaccination of hepatitis B to achieve more success of vaccination.