| The paper consists of three parts:isolation of human cytomegalovirus (HCMV) from infants and epidemiological investigation of HCMV infection in infants in Hunan province;determination of the envelope glycoprotein B(gB) genotype of the HCMV isolates;analysis of drug-resistance of the clinical isolates.1.Urine samples(HCMV DNA copy number＞103～107/ml) from 0～1-year-old infants with clinical symptoms were inoculated into confluent monolayers of human embryonic lung fibroblasts.The serum IgG and IgM responses of the infants and their mother were analyzed.And the data on the blood routine,the biochemical indices and the clinical symptoms of the infants were also taken into account.It was found that congenital infection with HCMV was liable to lead to neuron trauma (mental retardation,deafness,usually accompanied by movement disorder) in the newborns and the HCMV-infected babies seemed to easily develop pneumonia and hepatitis as well as nephritis,enteritis and anemia.2.To explore the relationship between genotypes distribution of gB and the occurrence of clinical symptoms,eight strains isolated from CPE-positive urine specimens were randomly selected.The genome DNAs were extracted and restriction fragment length polymorphisms (RFLP) were analyzed according to the results of nested polymerase chain reaction(nPCR).The UL55 genes of the isolates were also sequenced and compared.The results showed that the gB1 genotype was predominant among the HCMV isolates from infants in Hunan province. The sequence similarity of the clinical strains is 98.5%.Compared with the AD169 and Towne strains,the sequence similarities are 94.8%and 97.0%,respectively.3.Drug-resistance of the clinical strains was explored.(1) performance-based method.It showed that all the clinically isolated strains were sensitive to Ganciclovir and Foscarnet Sodium,but Huangqi had no effect on the replication of HCMV in the cell culture.(2) UL54 genotype method.Sequence analysis showed that the amino acid mutations mainly occurred in the section between theⅣ～δC zone andⅡ～δC zone. |
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