Pretection Of Propofol In Ischemia-reperfusion Injury Of Spinal Cord

Objective: An spinal cord ischemia model was established in New Zealand rabbits to investigate the protective effects of propofol on spinal cord ischemia-reperfusion injury (IRI) in terms of spinal histological morphology, neuroethology and the expression of oxygen free radicals, hypoxia inducible factor-1α(HIF-1α).Methods: The present study began with the observation of neurological behavior assessed by Tarlov scoring system and the spinal histological morphology changes of the spinal cord lumbar segments (L4-6) 48h after reperfusion. The feasibility of arterial injection of propofol was assessed. Then, propofol was applied to reperfusion rabbits by different methods (arterial or venous injection), the content of MDA and SOD in blood and spinal tissue and the expression of HIF-1αat different time points after the reperfusion were calculated, to evaluate the effects of propofol on the expression of oxygen free radicals and the protective mechanisms of propofol in the spinal cord ischemia-reperfusion injury.Results:⑴Content with group IR+PA(2.75±1.035) and IR+PV(1.50±0.925)(P﹤0.05) ,the Tarlov score is higest in all the group.Edema, focal or extensive necrosis, hemorrhage, swelling of nerve cells, and disappearance of nucleus were observed in IR,IR+FV and IR+FA group. In IR+PV and DR group, scarce normal nerve cells, karyopyknosis, nuclear lysis, vacuoles around nerve cells and Nissl bodies were observed. More normal as well as Nissl bodi after reperfusion (P<0.05);③No statistical differences were observed 6h after reperfusion (P>0.05);④Less MDA was obtained in IR+PA group than in IR group 24h after reperfusion(P<0.05). IR+PV group showed stronger activity of SOD than IR group 24h and 48h after reperfusion (P<0.05). The activity of SOD in IR+PA group increased significantly than in IR group (P<0.01).⑵: Weak HIF-1αprotein were observed in normal spinal cord. In IR+PV group, its expression increased significantly 48h after reperfusion. In IR+PA group, its expression reached the peak 48h after reperfusion and then declined.es were observed in IR+PA group.⑶Content of MDA and SOD in plasma and spinal cord tissue:①No statistical differences were observed between the samples taken 20min before and after ischemia (P>0.05);②More MDA and less SOD in IR group was obtained than in IR+PA group 20min and 6hConclusion: Arterial injection of propofol can reduce the content of oxygen free radicals in plasma and spinal cord tissue and increase the expression of HIF-1α. It plays an important role in the protection of spinal cord ischemia-reperfusion injury.Propofol plays protective role against IRI in the spinal cord of rabbit, and the enhancement of HIF-1αexpression might be involved in the protection induced by propofol.