Protection Of Hepatocyte Growth Factor In Rat's Transplantated Lung From Ischemia Reperfusion Injury

Lung transplantation has become the most effective therapeutic measure for chronicity pulmonary tuberculosis at the final stage, such as pulmonary fibrosis, primary pulmonary hypertension, emphysema, bronchiectasis and so on. While lung transplantation ischemia reperfusion injury(IRI) is the main cause of the transportation failure and patients'death at the earlier period. Therefore, how to lessen ischemical reperfusion injury has been the major point at the very present study period. During the patho and physio process of ischemia reperfusion, rapidly increasing oxyradicals and accumulation of Ca2 + among cells may lead to the apoptosis.Hepatocyte growth factor HGF has extreme effection on enhancement of pulmonary epithelial cells and suppression on IRI which leads to apoptosis pulmonary epithelial cells. Hence, we make full use of antiapoptosis of HGF which can alleviate the pulmonary I/R injury. This whole study consists of two parts as follows:1. To establish rat orthotopic left pulmonary allograft transplantation by improving the"cuff-like"vessel anastomosis technique.Objective To establish a more stable and effective rat orthotopic left pulmonary allograft transplantation models.Methods 40 rats were randomly divided into two groups: donors group and recipients group. Pulmonary arteries and veins were anastomosed by traditional cuff technique.Bronchi was reconstructed by stent techniqueResults 20 cases of rat orthotopic left pulmonary allograft transplantation was finished successfully. The total time of surgical procedure was (65±4)min. The operation successful rate was 85%. By blood gas and pathologic study we successfully found the typical lung ischemia reperfusion injury model.Conclusion It is an economical and successful model in highly imitating clinic lung transplantation, which can be used in lung ischemia reperfusion injury, 2. Protection of Hepatocyte Growth Factor in Rat's Lungs PreservationObjective To establish a more stable and effective rat orthotopic left pulmonary allograft transplantation models. To discuss the protection and mechanisms of hepatocyte growth factor in transplantated rat's lungs from ischemia reperfusion injury.Methods This study was divided into three groups: sham operation control group(A group),empty control group(B group) and HGF group(C group). Every group had 8 cases.The harvested lung blocks were flushed and stored in 4℃low-potassium-dextran and glucose (LPDG) solution for 4 hours in B group. 4℃LPDG solution which contains HGF(10ug/100ml)was used in C group. The experiment was over after flushing the transplanted lung 2h. The transplanted lung tissue wet-to-dry (W/D) ratio and activity of myeloperoxidase (MPO) were measured. TNF-αwas detected by ELISA. C-met were detected by immunohistochemical staining. Bcl-2 mRNA was detected by RT-PCR. Apoptosis is detected by TUNEL.Results Comparing with the empty control group, obviously, the degree of lung tissue injury in the HGF group reduces, and the lung tissue wet-dry weight ratio, MPO activity, TNF-αalso decrease (P <0.01). Moreover, the expression levels of c-met, Bcl-2 mRNA increased significantly (P <0.01), too.Conclusion HGF can effectively reduce IRI and further improve transplanted lung functions.The protection mechanism is probably associated with the stimulation of c-met and bcl-2 expression.