| Objective:To investigate the effect of Microtubule associated protein1light chain3on ischemia-reperfusion during rats lung-transplant through orthotopic lungtransplantation model. To provide new ideas for lung preservation inischemia-reperfusion injury.Methods: All rats in group were operated for orthotopic left lung allografttransplantation by using non-vessel endothelium cuff technique.The models couplesof SD rats were divided into control group (LDP group, n=14) and experimentalgroup (3-MA group, n=13) randomly. Systemic PvO2were determined in bloodsamples, The tissue sample taken from the left lung was checked for the expression ofLC3, the levels of the Methyl Di Alde Hyde (MDA), myeloperoxidase (MOP) activeand wet-to-dry weight ratio compared were detected. Histopathological changes andthe ultrastructural assessment of pulmonary were examined.Results:The3-MA group showed significantly higher levels of PvO2(mmHg) incomparison to LPD group；The levers of W/D were significantly decreased in3-MA group than LDP group； MDA content were significantly decreased in3-MAgroup than LDP group；MOP activity were significantly decreased in3-MA groupthan LDP group；group3-MA of LC3protein expression decreased in the groupLDP；Pathological changes: Alveolar cell swells and thickens, pulmonary edema,inflammatory cells and neutrophilc granulocyte invasion. Group3-Ma were betterthan group PLD.Conclusion: Microtubule associated protein1light chain3(LC3）related to lunginjury index on lung ischemia-reperfusion injury after lung transplantation in rats,Down-regulating expression of LC3can reduce lung ischemia reperfusion injury. |
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