Expression Of HSP27 In Postsynaptic Density Of Temporal Lobe Epilepsy Model

Objective:To investigate the expression of the heat shock protein(HSP27) in the postsynaptic density(PSD) of temporal lobe eplepsy(TLE),which would provide evidence for the relationship between HSP27 and epleptogenisis of TLE.Methods:In this study,the Sprague-Dawley rats were randomly enrolled in Normal group(Norm) and experiment group.The model of TLE was induced by lithium-pilocarpine in the experiment group.And the rats were further divided into TLE group and Non-TLE group based on recurrent spontaneous seizure or not in the next 30 days.PSD fractions were gradually obtained using sucrose gradient centrifugation abstraction.We semi-quantitatively analyzed HSP27 in PSD proteins from three groups using western blotting.Then we analyzed the expression of HSP27 in the PSD of CA1 area by pre-embedding immunogold electron microscopy.Results:1,The model of TLE:Status epilepticus were induced at the achievement ratio of 91.4%.The number of TLE rats is 32(45.7%),The number of n-TLE rats is 18(25.7%)2,The analysis of PSD purity:Two postsynaptic marker molecules NMDA receptor-1 and PSD-95 were expressed in P2,synaptosome and PSD fractions,but synaptophysin(presynaptic marker) was not expressed in PSD.3,Expression of HSP27 in PSD detected by western blotting:HSP27 were positively expressed in PSD of TLE,n-TLE and Norm group,the expressive levels of them were descending.Comparing to the normal group,the expressive levels of HSP27 in TLE group and those in n-TLE group were higher apparentely(P＜0.05),Comparing to the n-TLE group,the expressive level of HSP27 in TLE group was higher obviously (P＜0.05).4,Expression of HSP27 in PSD of hippocampus CA1 area detected by immunogold electron microscopy:HSP27 gold particles existed in the hippocampus CA1 area PSD of TLE,n-TLE and Norm group.Comparing to the normal group,the gold particles of PSD in TLE and n-TLE group were higher apparentely(P＜0.05),Comparing to the n-TLE group,the gold particles of PSD in TLE group were higher obviously(P＜0.05)Conclusions:HSP27 of PSD may participate in the epleptogenisis of TLE.