| Objective:At present,there were about 230 million couples of childbearing age people,the incidence of infertility was 15%-20%,that was to say there was at least 10 million home plagued by reproductive problems.According to the State Health Department survey data show that the infertility in patients with an upward trend,at present there was my country at 5.2 percent of married couples have to face the reality of infertility,fertility was still the problem of our country a variety of important tragicomedy one of the sources. Even in large and medium-sized city,because people will not have much fertility and divorce.According to the National Research Center of Beijing's 1000 divorce statistics,one of about 30%of people are because of the reasons children should not cause divorce.More liberal ideas advanced in Beijing like this,imagine the other regions on.According to statistics,the incidence of infertility in women of childbearing age about 15%to 20%,of which 50%of the reasons for the woman,the man 30%of the reasons,the reasons for both men and women accounted for 10%of those who did not identify the cause of about was 10%.Therefore,the study of infertility was necessary.At present, about Kidney infertility caused by experimental research and more clinical reports,the more in-depth study.However,due to deficiency regarding the experimental study of infertility rarely reported,so deficiency of this topic starting from the temper of infertility research must have practical significance and clinical significance.Methods:Choose healthy wistar male rats 40,adaptive feeding week to males were randomly divided into 4 groups:normal group,model group, WuZiYanZong pill group Sijunzi Soup group.Normal control group are not given any stimulus,and the rest of the group tired and cold diarrhea over law (add swimming Act administered rhubarb) rat model of Spleen Qi Deficiency. Model after the completion of five WuZiYanZong pill Group and Sijunzi soup were 21 days for delivery,and then measured the rats live sperm rate and density,to detect the serum gastrin,D-xylitol concentration,flow cytometry Detection of rats in each group of spermatogenic cell apoptosis rate,the percentage of spermatogenic cells at all levels,statistical analysis of the significance of the target changes.Results:(1) determination of sperm parameters:comparison with the normal group,model group,WuZiYanZong pill group sperm density,sperm viability was significantly lower(P＜0.01),Sijunzi soup group rats significantly decreased sperm density(P＜0.01),live sperm rate had no obvious abnormalities(P＞0.05).Compared with model group, WuZiYanZong pill group sperm density,sperm viability were not significantly different(P＞0.05);Sijunzi soup group rats live sperm and sperm density was significantly higher(P＜0.01),show that Sijunzi soup can significantly enhance the number of rat sperm and live sperm rate.Compared with WuZiYanZong pill group,rats Sijunzi soup group sperm density, sperm viability were significantly higher(P＜0.05),shows that Sijunzi soup rat model to improve the sperm density and sperm viability was stronger than WuZiYanZong pill.Rats in each group the rate of sperm deformity was no significant difference between the comparison,that is,Qi Deficiency on rat sperm abnormality rate have little effect.(2) serum gastrin,D-xylitol concentration determination:comparison with the normal group,model group,and Zi Yan Zong Wan five serum gastrin,D-xylitol concentrations were significantly lower(P＜0.01),and Sijunzi soup group no significant difference between groups(P＞0.05).Compared with model group, WuZiYanZong pill group serum gastrin,D-xylitol concentration had no significant difference(P＞0.05);Sijunzitang serum gastrin,D-xylitol concentration rose significantly high(P＜0.01).Compared with WuZiYanZong pill group,Sijunzitang serum gastrin,D-xylitol concentrations were significantly higher(P＜0.05),note the improvement Sijunzi soup group deficiency rat model was superior to the role of WuZiYanZong pill group.(3) the rate of spermatogenic cell apoptosis,the number of spermatogenic cells at all levels,the determination of the percentage.①the rate of spermatogenic cell apoptosis:comparison with the normal group,model group and WuZiYanZong pill group spermatogenic cell apoptosis rate was significantly increased(P＜0.05),while Sijunzi soup group no significant difference between groups(P＞0.05).Compared with model group, WuZiYanZong pill group spermatogenic cell apoptosis was no significant difference(P＞0.05);Sijunzi soup group rat spermatogenic cell apoptosis was significantly lower(P＜0.05).Compared with WuZiYanZong pill group,rats Sijunzitang spermatogenic cell apoptosis was significantly lower(P＜0.05), note Sijunzitang spermatogenic inhibitory role in apoptosis rate was superior to group WuZiYanZong pill.②the number of spermatogenic cells at all levels,percentage:Compared with the normal group,model group 1C (haploid,including sperm cells and sperm) a significant reduction in the percentage(P＜0.01),model group and WuZiYanZong pill group 4C (tetraploid,including primary spermatocytes and G2 spermatogonia period) significantly reduced the percentage(P＜0.05),while the group Sijunzi soup group spermatogenic cells at all levels there is no significant difference in the percentage(P＞0.05).Compared with model group,and WuZiYanZong pill Sijunzi soup group 1C rats significantly higher percentage(P＜0.05);Sijunzi soup group 4C rats significantly higher percentage(P＜0.05).WuZiYanZong pill group,rats Sijunzitang 4C significantly higher percentage(P＜0.05).Conclusions:(1) Spleen Qi Deficiency can reduce the serum gastrin, D-xylitol content indirectly affect the reproductive function.(2) Spleen Qi Deficiency can reduce sperm count,sperm viability and affect their reproductive function.(3) Spleen Qi Deficiency can reduce the number of spermatogenic cells at all levels,resulting in sperm production and the impact of barriers to their reproductive function.(4) Spleen Qi Deficiency can be through the promotion of male rat spermatogenic cell apoptosis and affect the occurrence of sperm development have an impact on the reproductive function.(5) Sijunzi soup can adjust the levels of serum gastrin,D-xylitol content,inhibited apoptosis of spermatogenic cells,adjust the number of spermatogenic cells at all levels,improve sperm quality and improve their reproductive function.(6) The experimental results show that Spleen Qi Deficiency can reduce male reproductive function,tonic temper can restore their reproductive function,and thus on the experimental verification of the Spleen Qi Deficiency and reproductive function.
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