The SiRNA Targeting BAK Inhibit Apoptosis Of DC That Induced By Glioma And The Mechanism

Object Dendritic Cells co-culture with glioma,To study the apoptosis of Dendritic Cells that induced by glioma.To study the vector expressing siRNA targeting BAK inhibit the apotosis of Dendritic Cells and to speculate the possible pathway of the apoptosis.Methods The vector that expressing siRNA targeting BAK was transfected into DC by the lipofectamine2000 and the cells stably expressing the siRNA were selected by G418.To choose the high performance vecteor that interference BAK.Then the DC that stably expressing the siRNA were co-culture with glioma and observe the apoptosis of DC.Investing the relate gene for example caspase3 caspase8 and the cytochrome c in endochylema,to speculate the possible pathway of the apoptopsis.Resuits When the DC2.4 co-culture with glioma lead to the apoptosis.When the vector were transfected into DC2.4,the expression of BAK were degraded(western blot:A:0.83,B:0.14,C:0.75,D:0.89,un-transfecte:0.92; RT-PCR.- A:0.42,B:0.08,C:0.39,D:0.46,un-transfecte:0.49 P＜0.05).When the DC2.4 that stably expressing siRNA target BAk co-culture with glioma,the expression of caspase 8 was not change(a:0.78 b:0.75 c:0.77,P＞0.05) to tmexpression siRNA one,but the expression of caspase 3 and the cytochrome C (caspase3:a:0.67 b:0.70 c:0.2;Cytc:a:0.70 b:0.71 c:0.17 P＜0.05)in endochylema was less than the unexpression siRNA one.Conclusion The glioma can induce the apoptosis of DC2.4.The pathway of apoptosis may be the mitochondrial pathway; The vector that expresse siRNA targeting BAk can degrade the apoptosis of DC2.4.