Study On The Flavonoids In Fruit Of The Catalpa Ovata G.Don And Volatile Oil In The Flowers

The flavonoids is a phenolic compounds distributing widely in nature, especially in plant kingdom. Recently, people pay more and more attention to them. Catalpa ovata G.Don belongs to the family of Bignoniaceae. Its plant has many functions, such as clearing-detoxication, and has been used as a traditional Chinese medicine for cirrhosis. But because it lack phlebitis with marked curative effect, it didn't recorded in pharmacopoeia. The major contents of this research are the flavonoids in the fruit of Catalpa ovata G.Don. and the volatile oil in the flowers. It will provide theory evidence for the development of pharmaceutical industry and cosmetic industry.It was proved that flavonoids was exist in the fruit of Catalpa ovata G. Don by pre-test. The extraction technology of flavonoids from the fruit of Catalpa ovata G. Don used traditional hot organic solvent method. The extraction rate of flavonoids optimized condition(ethanol concentration, extraction temperature, ratio of solid to liquid and extraction time) were determined by orthogonal design. The result showed that the optimized conditions was obtained as: ethanol concentration is 60%; extraction temperature is 70℃; the ratio of solid to liquid is 1:20; extraction time is 2.0h and extract yield is 4.83%. Contrasted with rutin, we studied the total content of flanovoids in different part of Catalpa ovata G. Don. ( fruits, leaves, flowers), by spectrophotometry. The results showed that the total content of flavonoids was highest in fruits.Flavonoid was purified with the AB-8 pocket adsorption resin, the adsorption rate was 93.98% , the desorption rate was 68.60%. Purification flavonoids was mainly contain flavone, flavonol, isoflavone. The result of thin layer chromatographic analysis showed that purification flavonoids may contain rutin.Antioxidan experiment showed that the flavonoids in the fruit of Catalpa ovata G. Don had good oxidation resistance activeness. The antibacterial experiment showed that the purification flavonoids had good inhibition to Bacillus subtilis and Bacillus thuringiensis.The essential oil in the flowers of Catalpa ovata G. Don were extracted by steam distillation and analyzed by using GC-MS, 32 compounds are identified. The compound which content surpass 5% were phenanthrene (27.21%), fluorine (7.71%), tetracotane (6.17%), tetracosane (6.05%).