| Objective:This study was to compare the osteogenic potential and biomechanical performance of Hollow Porous Titanium Prostheses(HPTP) loading with BMP slow-release combination and Hollow Porous Titanium Prostheses(HPTP) loading with BMP slow-release combination and autografting BMSCs in rabbits;and to discuss the feasibility of enhancing the integration of Hollow Porous Titanium Prostheses(HPTP) with the bone around it.We hope the results will provide an experimental basis for the study of hollow porous artificial joint prostheses and other bone-regeneration implants,and will be beneficial for the design and improvement of biological artificial joint.Methods:We designed a HPTP with many holes(2mm in diameter).The internal surface and part of superficies extema which will be implanted in bone of the prostheses were spray coated with hydroxyapatite(HA).There were 2 groups in this research: Hollow Porous Titanium Prostheses(HPTP) loading with BMP slow-release combination(group A),Hollow Porous Titanium Prostheses(HPTP) loading with BMP slow-release combination and autografting BMSCs(group B).Eighteen New Zealand white rabbits(4～6 months old) were used totally,9 in each group.All the implants were placed in the femoral condyles of rabbits transversely.3,8,12 weeks after operation,we took out corresponding specimens,and studied the bone formation around and within the prostheses with common light microscopy, fluorescence microscopy and software for norphometry.Besides,6 specimens in each group were used for biomechanical tests at the 12th week point.CHISS2004 software was used for statistical analysis,and measurement data were expressed in the form of mean value±standard deviation((?);±s).Results:There were no obviously different in outward appearance between Group A and B. 3 to 12 week after operation,bone tissue regenerate quickly,especially where the BMP slow-release combination was.Bone tissue could grow into the holes on porous prostheses,make the bone inside integrate with the bone outside.The morphometry results show that bone regeneration rate of group B is significantly higher than group A at 8 and 12 week after operation,p＜0.01;but the difference has no significance at 3 week after operation,p＞0.05.In biomechanical tests,there was no statistical difference between the pull-out forces of group B and group A.Conclusion:1.Hollow Porous Titanium Prostheses(HPTP) loading with BMP slow-release combination and autografting BMSCs offer better conditions for bone regeneration than Hollow Porous Titanium Prostheses(HPTP) loading with BMP slow-release combination.,which can improve integration of prostheses and bone tissue significantly.2.BMSCs has no effect on the pull-out force of Hollow Porous Titanium Prostheses(HPTP) loading with BMP slow-release combination3.The idea for HPTP is feasible,Bone tissue could grow into the holes on porous prostheses,make the bone inside integrate with the bone outside.4.HA coating of the HPTP can integrate with bone tissue very well.Our experiment in vivo shows that Hollow Porous Titanium Prostheses(HPTP ) loading with BMP slow-release combination and autografting BMSCs offer better conditions for bone regeneration than Hollow Porous Titanium Prostheses(HPTP) loading with BMP slow-release combination.It offers elementary experiment support for the design of artificial joint in the further. |
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