| Luminescence analysis is the abbreviation of molecular spectrum,composing of molecular fluorescence,phosphorescence and chemluminescence analysis(including bioluminescence).Fluorescence emits when the electrons transfer from the fisrt excited single state to the base state.Since 1867,Goppelsroder has carried out the fluorescence analysis for the first time in history,in addition to the general fluorescence analysis,such as the nonfluorescence substance being transferred into fluorescence ones which can be determined,fluorescence quenching method,energy transferring method and so on, with the introduction of laser,computers and advancement of electronics,synchromous fluorimetry,deivative fluorimetry,time-resolved fluorescence,fluorescence polaruzation,fluorescence probe and other methods have greatly developed, accelerating the advent of all kinds of new fluorescence instruments.Now,the fluorescence analysis has being concerned in biological reaction control,biosensor, pharmaceuticals minitor,biochemical analysis and multi-content in complicated system.And it has been developed into an important effective spectrum analusis technology.There are several characteristics of fluorescence analysis,which are high selective,less sampling,simple and rapid.The paper Reviews the application of fluorescence in pharmaceutical.In this paper,we determine the amount of pharmaceutical by derivative fluorescence spectroscopy with complex reaction.The thesis consists of three parts:Part 1 Study on Determination of Cephalexin in Capsules by Fluorescence Chromogenic ReactionCephalexin,a nonfluorescent substance,is transformed into a highly fluorescent compound(λex=355nm,λem=438nm) with metal ions Au(Ⅲ) in pH(7.0～7.2) medium. Thus,a photochemical-fluorimetric method for the determination of cephalexin was developed with a detection limit of 0.14 mg·L-1.The linear relationship between the fluorescence intensity and the concentration of cephalexin is over the range of 0.40 20.0 mg·L-1.The relative standard deviation for the determination of 2.0 mg·L-1 cephalexin is 2.48%(n=11).This method has been successfully applied to the determination of cephalexin in capsules.Part 2 study on Inhibitory Kinetic Fluorimetric in Pharmaceutical analysisChapter 1 Determination of Ceftriaxone Sodium in InjectionBased on the inhibitory effect of ceftriaxone sodium on the oxidation reaction of K2S2O8 for berberine in acid medium,an inhibitory-kinetic fluorimetric method is developed for the determination of ceftriaxone sodium.The detection limit for ceftriaxone sodium is 3.8×10-8 g·mL-1 and the relative standard deviation is 1.29%. The linear range of the determination is 1.0×10-6～1.3×10-5 g·mL-1.This method is simple,rapid,and sensitive,and can be used for the determination of ceftriaxone sodium in pharmaceutical formulation.Chapter 2 Determination of Piroxicam in TabletsAn inhibitory-kinetic fluorimetric method is found for the determination of Piroxicam.The proposed method is based on the inhibitory effect of Piroxicam on the reaction of K2S2O8 oxidation of berberine catalyzed in acid buffer solution medium. The detection limit for Piroxicam is 4.5×10-8 g·mL-1 and the standard deviation is 3.12 %.The linear range of the determination is 1.0×10-7～2.0×10-6 g·mL-1.This method is simple,rapid,and sensitive,and can be used for the determination of Piroxicam in troches.Chapter 3 Determination of Cefoperazone SodiumBased on the inhibitory effect of cefoperazone sodium on the oxidation reaction of K2S2O8 for berberine in acid medium,an inhibitory-kinetic fluorimetric method is developed for the determination of cefoperazone sodium.The detection limit for cefoperazone sodium is 7.0×10-8 g·mL-1 and the relative standard deviation is 2.32%. The linear range of the determination is 1.0×10-6～1.3×10-5 g·mL-1.This method is simple,rapid,and sensitive,and can be used for the determination of cefoperazone sodium in pharmaceutical formulation.Part 3 Study on the Determination of Amikin with Resonance Light Scattering MethodTo establish a new method for determination of Amikin.The method is based on the interaction of Amikin and Eosin Y in the presence of SDS and in the Britton-Robinson buffer solution(pH=5.5).The interaction results in an resonance light scattering(RLS).At the maximal wavelength of 525 nm,the enhanced RLS intensity is proportional to the concentration of Amikin in the range of 4.0～20.0μg·mL-1.The detection limit is 0.011μg·mL-1 and the relative standard deviation of Amikin is 3.23% for 4.0μg·mL-1(N=11).The proposed method is rapid,simple,sensitive and can be successfully applied for the determination of Amikin in pharmaceutical formulations.
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