Studu On Application Of Luminescence Analysis In Cephalosporin Antibioticsical Detection

This thesis consists of two parts: review and research report. The review involves the application of fluorimetry and chemiluminescence in pharmaceutical. The research report is focused on the study of the determination of inhibitory kinetic fluorimetric analysis and FIA-chemiluminescence analysis in antibiotics.1. FIA-Chemiluminescence Determination of Cefotaxime SodiumA new method for determination of cefotaxime sodium was developed using flow injection chemiluminescence analysis based on that cefotaxime sodium could greatly enhance the emission intensity of the chemiluminescence reaction of K₃Fe(CN)₆ and luminol in alkaline medium. The relative chemiluminescence intensity is proportional to cefotaxime sodium concentration over the range 2.4×10^-63.0×10^-4 g/mL with a detection limit of 6.3×10^-7 g/mL. 9 times of parallel determination of 3.0×10^-4 g/mL cefotaxime sodium show that the relative standard deviation (RSD) is 1.0%. It has been satisfactorily applied to determine cefotaxime sodium in injection.2. Determination of Cefoperazone Sodium with Chemiluminescence catalyzed by Nanogold ParticlesIn alkaline medium, the system of nanogold catalyzed silver nitrate-luminol has a very strong chemiluminescence. Cefoperazone sodium can inhibit emission of this system. A new analytical method for determination of cefoperazone sodium was established based on this. In the optimum conditions, the linear range was 5.0×10^-79.0×10^-4 g/mL with the detection limit of 1.0×10^-7 g/mL. The relative standard deviation (RSD) was 1.6% for 3.0×10^-6 g/mL cefoperazone sodium (n=11). This method is simple, rapid with broad linear range. It has been satisfactorily applied to determine cefoperazone sodium in injection.3. Determination of Ceftazidime by Flow Injection ChemiluminescenceIn alkaline medium, gold nano-particles could catalyze the chemiluminescence (CL) of the luminol-potassium permanganate system, and ceftazidime could enhance CL intensity of this system. Based on this, a flow injection CL method for determination of ceftazidime was established. In optimized condition, the linear range of determination was 3.0×10^-85.0×10^-5 g/mL with the detection limit of 1.0×10^-8 g/mL. The relative standard deviation (RSD) was 2.0% for 4.0×10^-6 g/mL ceftazidime (n=11). It has been satisfactorily applied to determine ceftazidime in injection. This method is simple and rapid with broad linear range.4. Inhibitory Kinetic Fluorimetric Determination of Cefoperazone Sodium in InjectionBased on the inhibitory effect of cefoperazone sodium on the oxidation reaction of K₂S₂O₈ for eosin-Y in weak acid medium, an inhibitory-kinetic fluorimetric method is developed for the determination of cefoperazone sodium. The linear range of the determination is 1.0×10^-62.0×10^-5 g/mL (r=0.9986) with the detection limit of 3.8×10^-8 g/mL. And the relative standard deviation (RSD) is 2.0%. This method is simple, rapid and credible, which can be used for the determination of cefoperazone sodium in pharmaceutical formulation.5. Inhibitory Kinetic Fluorinetric Determination of Cephradine in CapsuleBased on the inhibitory effect of cephradine on the reaction of K₂S₂O₈ for titan yellow in alkaline medium, an inhibitory-kinetic fluorimetric method is developed for the determination of cephradine. In the optimum conditions, the linear range was 5.0×10^-71.0×10^-5 g/mL with the detection limit of 5.6×10^-8 g/mL. The relative standard deviation (RSD) was 1.1% for 5.0×10^-6 g/mL cephradine (n=11). This method is simple, rapid and credible, which can be used for the determination of cephradine in pharmaceutical formulation.