| In our country,lung cancer has high morbidity and mortality all tumors,become the fastest morbidity and mortality and serious harm to human health and life of malignant tumors.In the anti-tumor process,the cell immunity induced by T-lymphocytes plays a very important role.Especially the tumor-cell killing function induced by lytotoxic T-lymphocytes,which acts as a critical role.When different T-lymphocyte subsets present abnormality quantity and function. It will result in the disorder of the immune function and killing decrease of anti-tumor ability.As a result,the evaluation of immune function of lung cancer Patient by T-lymphocyte subset is more valuable clinically.Being a cytokine with great biologic activity,the anti-tumor and cytotoxic functions of TNF-βwere noticed initially,then its anti-viral activities were proved later.Having better anti-tumor functions than TNF-αand a great potential of being drugs,TNF-βis considered as one of the effective products of immunotherapy.In order to investigate the changes of TNF-βand its producing cell T-lymphocytes in the serum of patients with lung cancer,and study their clinical significance,we determined the TNF-βlevels in serum and T-lymphocyte subsets in peripheral blood of 65 patients with untreated primary lung cancer and 20 controlled healthy subjects,compared them based on lung cancer types and stages,and analyzed their change regularity and clinical significance.Objective:To investigate the clinical significance and changes of serum tumor necrosis factorβ(TNF-β) and peripheral blood T-lymphocyte subset in patients with lung cancer.We offer a new theoretical evidence for clinical analysis the immunological function of patients with lung. Methods:We extracted serum samples from 20 healthy subjects and 65 untreated patients with primary lung cancers.Changes of serum TNF-βdetected using ELISA techniques,and T-lymphocyte subset in peripheral blood measured by the flow cytometer using bicolor factorial method in the 65 lung cancer patients were compared with those in the 20 healthy subjects.Results:The level of serum TNF-βin lung cancer patients was obviously lower than that in healthy subjects,not only in various histological types but also on different clinical phases.T lymphocyte CD3+,CD4+,CD8+ and CD4+/CD8+ has the same or similar statistic tendency to TNF-β.Marked decrease of serum activated T-lymphocyte,which is the main carrier of the cellular immunity,leads to depression of cellular immunity in patients with lung cancer.Because of the decrease of the main producer, the level of serum TNF-βdescends in lung cancer patients,which can lead to depression of physiological activity of TNF-βand its anti-tumor capability.Conclusion:1.In the peripheral blood of lung cancer patient,the level of all T-lymphocyte subsets decreases,more obviously in higher clinical phase and more malignant cases.The decrease of T-lymphocyte inevitably leads to reduction of immune faction of patient.2.The decrease of T-lymphocyte lead to the reduction TNF-β,which consequently result in the decrease of anti-tumor ability and immune function.3.The levels of T-lymphocyte subsets and serum TNF-βdecrease and they have a positive correlation.They have the same similar statistic tendency.Both have the same or similar statistic tendency to be obviously lower than that in healthy subjects,not only in various histological types but also on different clinical phases.4.The level of peripheral blood T-cell subset and serum TNF-βreduce in patients with lung cancer.The clinical significance of the activity study may be used as an objective index and treatment.
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