The Effects Of BFGF On Neural Regeneration And Recovery After Traumatic Brain Injury In Mice

Traumatic brain injury is a common disease of the central nervous system,which cause significant harm to human health.Although with the continuous development of medical conditions,the decrease in mortality and disability,the recovery of brain injury is still not satisfactory.How to reduce post-traumatic re-injury,disability rates and improve the cure rate is one of the important research direction to prevent and treat traumatic brain injury.After the occurrence of brain damage,some cells necrosis and other cells apoptosis.As a programmed cell death,apoptosis was able to participate in the process of re-injury after brain injury,caspase-3 was considered the most critical factor to promote the apoptosis.Astrocytes secrete a variety of factors,which can promote the damage repair and nerve regeneration.Basic fibroblast growth factor is an important neurotrophic factor,and it can promote neuron survival and neurite growth, which play a positive role on repair of nerve cells and astrocytes.Thus bFGF may become a source of drugs for treatment of brain injury.Objective To establish a traumatic brain injury model and explore the effect of exogenous basic fibrolast growth factor on neural regeneration after traumatic brain injury in mice.Methods Traumatic brain injury(TBI)model was induced in mice by using freely dropping coup techniques.The animals were divided randomly into three groups including bFGF group,saline control group and injured control group.After TBI bFGF group was injected 8μg/kg bFGF per day by intraperitoneal,saline group was injected with the amount of normal saline,injured control group without any other treatment,and keeping with the other groups.Immunofluorescence and western blot were employed to determine alternations in caspase-3,GFAP levels in injuried cortex and hippocampus after TBI,the double label was used to detect alternations in the coexpression of positive cells of NF-200 and caspase-3 by fluorescence microscope.The infarction area was measured using HE staining combined with the image analysis system software.The motor function and learning memory abilities were measured by the pole experimentation and Y-maze test.Results The expressions of caspase-3 of cerebral cortex and hippocampus in bFGF group were lower than saline group(P＜0.05).In addition,the expressions of GFAP of cerebral cortex in bFGF group were higher than saline group(P＜0.05),and there was no difference on expression of caspase-3,GFAP between injured control group and saline group(P＞0.05).Learning and memory abilities and infarction area for bFGF group comparing with that of the injured control group and saline group,there was a significant difference(P＜0.05),and saline group had no improvement than the injured control group(P＞0.05).Conclusion bFGF may decrease the expressions of caspase-3,inhibit the neuronal apoptosis cells in cerebral cortex and hippocampus,and increase activation of astrocytes and the gumnosis of cerebral cortex after TBI.bFGF can also improve the neurologic functions and reduce the incidence of sequelae after TBI.