Controlled Experiment Study Of Quinolone-induced Articular Cartilage Injury With MRI And Pathology

Background: Quinolones belong to chemosynthesis antibacterials can selectively inhibit the DNA gyrase of bacterium. Quinolones are rapidly bactericidal and have an extended antimicrobial spectrum, and they have advantageous pharmacokinetic properties such as excellent penetration into many tissues. Thus, they have been showed to be effective in the treatment of a large variety of bacterial infection. However, all kinds of drug adverse reaction (ADR) has been observed in clinical therapy, such as photoxicity, heart toxicity, articular cartilage toxicity, ADR in digestive system and central nervous and so on. Especially, people gradually pay more attention to the articular cartilage toxicity of QNs since many animal experiments show a major drawback of QNs is the potential to induce arthropathy in juvenile animals, which cue that QNs may have the same articular cartilage toxicity to children, arthropathy has been reported in rare case reports in children. Thus, the toxicity potential has led to important restrictions of the use of QNs in children. On the other hand, many investigations showed no evidences of chondrotoxicity with QNs in pediatric patients and QNs have some ascendancy to treat many ailments. So in some occasions, many children have received treatment with QNs.Objectives: Applied MRI to observe the quinolone-induced articular cartilage injury in juvenile rabbit knees. Contrast the performance of MRI and pathology , to summarize the performance of MRI in the quinolone-induced injured articular cartilage ,in order to provide imageology guidance for clinical.Methods: 20 healthy juvenile rabbits (32 days after birth) were equally divided between male and female; rabbits were randomly assigned to blank control group and experimental group (each including 10 animals).The experimental group were given ciprofloxacin 300mg/kg d per os once a day , the control group were given normal saline , consecutive 10 days administration. Scan the knees of the two groups before and after the administration one week. The completion of magnetic resonance scan within 24 hours taking the knees of two sets of experimental animals, and femoral condyle and tibial plateau cartilage biopsy were to do. Specimens were fixed with 10% formalin 48 hours and decalcified with 10% nitric acid 5 days, and then step by step alcohol dehydration, paraffin-embedded sections, HE conventional staining, the changes of the cartilage were observed by biological microscope in×40,×100 magnification. Measure the thickness on the same site of bilateral femoral articular cartilage of the two groups with universal video image device to draw the normal range and the value of the experimental group. Analysis the magnetic resonance imaging and pathology results of the two groups of animals and using SPSS 13.0 statistical software package for data analysis, measurement data is handled by two-sample t-test,enumeration data using paired fourfold tableχ2 test, P<0.05 considered there is no statistical significant difference.Results: 1.Make a animal modal of quinolone-induced juvenile rabbit cartilage injury successfully.2.Contrast the MRI and pathological results of the quinolone-induced articular cartilage injury in juvenile rabbit knees, and analysis objective, different degrees of injury or thinning in the knee cartilage tissues of experimental group rabbits can be seen in optics microscopy, and the corresponding performance can be seen in MRI images.Conclusion: MRI can show the earlier changes such as thinningz of articular cartilage of quinolone-induced juvenile animals cartilage injury.