The Effect Of Bone Marrow Mesenchymal Stem Cells Transplantation On Type 2 Diabetic Cardiomyopathy Rats

Purposes:To explore therapeutical effect and the mechanism of MSCs transplantation improving type 2 diabetic cardiomyopathy models.Method:Rats MSCs were isolated from rat bone marrow from femurs and tibias under sterile condition. Human MSCs were isolated from normal human and diabetic bone marrow under sterile condition. The surface markers of MSCs, including CD34,CD44, CD45,CD71, CD105, were detected by flow cytometry; detecting proliferation and apoptosis of MCSs of normal human under high glucose and high palmitinic acid cultivation using MTT and Annexin/PI fluorescence staining, and detecting proliferation and apoptosis of MCSs of type 2 diabetic patients under low glucose DMEM cultivation using PI fluorescence staining; The type 2 diabetic cardiomyopathy model of Wistar rats was established by injection of STZ intraperitoneally after feeding with forage including sugar and egg yolk 4 weeks, and treating the type 2 diabetic cardiomyopathy model by transplanting Rats MSCs; Comparing heart function by echocardiogram, comparing content of glucose, triglyceride and insulin in plasma, comparing pathology of the cardiac muscle by HE staining, comparing mRNA expression of PPARαand PPARγby RT-PCR.Result:Proliferation of human MSCs decreased, apoptosis of human MSCs increased under high glucose and high palmitinic acid cultivation. The difference of Proliferation and apoptosis between MSCs of diabetic cardiomyopathy patients and MSCs of normal persons is not obvious. heart function of diabetic cardiomyopathy model rats is improved after MSCs transplantation treating; It showed that cardiac muscle cell arrayed regularly without missing using HE staining. content of glucose is decreased, and insulin is decreased after MSCs transplantation treating; mRNA expression of PPARαand PPARγis increased after MSCs transplantation treating.Conclusion:High glucose and high palmitinic acid environment influences growth of human MSCs, and growth of MSCs of diabetic recovers away from high glucose and high palmitinic acid environment; Rats MSCs transplantation improves heart function ,blood glucose and pathology changes of the type 2 diabetic cardiomyopathy model; Rats MSCs transplantation increases the mRNA expression of PPARαand PPARγ.