| Objective To investigate whether or not caffeine can improve cisplatin-induced apoptosis in treatment of osteosarcoma cell line U2-OS. Methods The osteosarcoma cell line U2-OS was incubated with different concentrations of cisplatin (0.2, 2, 5, 10, 20μg/L), caffeine (0.2, 2.0mmol/L) and caffeine+cisplatin for 24h, 48h, 72h. With 3-(4,5-dimethylthiazol-2-y1)-2,5-diphenyltetrazolium bromide (MTT) assay the cytotoxicity was observed;the proliferation of U2-OS cell on the influence of different concentration of cisplatin and caffeine at different time point were analyzed by blow cytometry (FCM); apoptosis of U2-OS cell observed under fluorescence microscope. Results The proliferation of U2-OS cell was inhibited. The apoptosis of osteosarcoma was enhanced as the concentrations of caffeine or cisplatin increased. When the cells were treated with the combination of caffeine and cisplatin, the apoptosis increased more prominently. Enhancement effect of caffeine in cisplatin-induced apoptosis of osteosarcoma was observed from three aspects. In particular, 0.2 micrograms/ml CDDP with 2 mM caffeine showed a considerable synergistic effect on osteosarcoma cell line U2-OS. Conclusion Caffeine had remarkable enhancement effect in cisplatin-induced apoptosis of osteosarcoma cell line U2-OS. Therefore, we assumed that caffeine was able to reduce the necessary dose of anti-tumor agent in some way. We stress that caffeine seems to be a very useful synergistic drug for causing lethality in osteosarcoma cell line U2-OS in combination with various DNA-damaging agents which are not effective on sarcoma cells. |
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